中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (2): 85-90.

• 原著:神经损伤修复保护与再生 •    下一篇

Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha- synuclein

  

  • 收稿日期:2011-09-29 修回日期:2011-12-28 出版日期:2012-01-15 发布日期:2012-01-15

Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha- synuclein

Tao Chen, Xiaoping Liao, Guoqiang Wen, Yidong Deng, Min Guo, Zhigang Long, Feng Ouyang   

  1. Department of Neurology, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China
  • Received:2011-09-29 Revised:2011-12-28 Online:2012-01-15 Published:2012-01-15
  • Contact: Xiaoping Liao, Chief physi-cian, Department of Neurol-ogy, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China liaoxp@163.com
  • About author:Tao Chen☆, Doctor, Chief physician, Department of Neurology, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China

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Abstract:

The specific and effective α-synuclein RNA interference (RNAi) plasmids, and the α-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 (HEK293) cells using the lipofectamine method. Using an inverted fluorescence microscope, α-synuclein proteins were observed to aggregate in the cytoplasm and nucleus. Wild-type α-synuclein proteins co-localized with mitochondria. Hematoxylin-eosin staining revealed round eosinophilic bodies (Lewy body-like inclusions) in the cytoplasm of some cells transfected with α-synuclein-pEGFP plasmid. However, the formation of Lewy body-like inclusions was not observed following transfection with the RNAi pSYN-1 plasmid. RNAi blocked Lewy body-like inclusions in the cytoplasm of HEK293 cells induced by wild-type α-synuclein overexpression, but RNAi did not affect the subcellular localization of wild-type α-synuclein in mitochondria.

Key words: RNA interference, α-synuclein gene, subcellular localization, inclusion, Parkinson’s disease, neural regeneration