中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (1): 54-59.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

Ultrasound microbubbles combined with liposome-mediated pNogo-R shRNA delivery into neural stem cells

  

  • 收稿日期:2011-09-25 修回日期:2011-12-23 出版日期:2012-01-05 发布日期:2012-01-05

Ultrasound microbubbles combined with liposome-mediated pNogo-R shRNA delivery into neural stem cells

Weixia Ye1, Xueping Huang2, Yangyang Sun1, Hao Liu1, Jin Jiang1, Youde Cao1   

  1. 1 Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China
    2 Department of Orthopaedics, Luzhou People’s Hospital, Luzhou 646000, Sichuan Province, China
  • Received:2011-09-25 Revised:2011-12-23 Online:2012-01-05 Published:2012-01-05
  • Contact: Youde Cao, Doctor, Professor, Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China cydcyj@163.com
  • About author:Weixia Ye★, Studying for master’s degree, Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China (working in Department of Gastroenterology, Luzhou People’s Hospital)

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Abstract:

In the present study, ultrasound-mediated microbubble destruction (UMMD) alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor (Nogo-R) shRNA plasmid (pNogo-R shRNA) into neural stem cells (NSCs). Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs.

Key words: ultrasound microbubble, liposome, neural stem cell, gene transfection, Nogo receptor, neural regeneration