中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (35): 2784-2792.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

跨膜肽聚乙二醇-脂质体可跨越血脊髓屏障及血脑屏障

  

  • 收稿日期:2012-08-15 修回日期:2012-11-07 出版日期:2012-12-15 发布日期:2012-12-15

Transactivating-transduction protein-polyethylene glycol modified liposomes traverse the blood-spinal cord and blood-brain barriers

Xianhu Zhou1, Chunyuan Wang1, Shiqing Feng1, Jin Chang2, Xiaohong Kong3, Yang Liu1,Shijie Gao1   

  1. 1 Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China
    2 School of Material Science and Engineering, Tianjin University, Tianjin 300072, China
    3 Medicine College, Nankai University, Tianjin 300071, China
  • Received:2012-08-15 Revised:2012-11-07 Online:2012-12-15 Published:2012-12-15
  • Contact: Shiqing Feng, M.D.,Professor, Chief physician,Doctoral supervisor,Department of Orthopedic Surgery, Tianjin Medical University General Hospital,Tianjin 300052, China fengsq321@sina.com
  • About author:Xianhu Zhou☆, M.D.,Associate chief physician,Department of Orthopedic Surgery, Tianjin Medical University General Hospital,Tianjin 300052, China

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Abstract:

Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts.Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fluorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells (MCF-7). Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.

Key words: liposomes, transactivating-transduction protein, polyethylene glycol, blood-spinal cord barrier;
blood-brain barrier, caudal vein, fluorescein isothiocyanate, rat, human breast adenocarcinoma
cells (MCF-7), cytobiology, neural regeneration