脊髓损伤
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Figure 4｜DUSP2 overexpression weakens the capacity of axon regeneration.
To acquire more information about DUSP2 function in axon regeneration, we constructed a UAS-dusp2 plasmids that overexpresses DUSP2 when co-transfected with CMV/Huc-GAL4-VP16 (Figure 4A). We co-microinjected Huc-GAL4-VP16/UAS-dusp2 plasmids and Huc-GAL4-VP16/UAS-mCherry plasmids into one-cell stage eggs. Imaging results showed that mCherry was expressed in neuronal somata and axons (Figure 4B). qRT-PCR results confirmed the overexpression of dusp2 (P < 0.0001; Figure 4C).
We then used single-cell electroporation to overexpress DUSP2 in M-cells in vivo. CMV-GAL4-VP16/UAS-dusp2/UAS-mCherry plasmids and CMV-GAL4-VP16/UAS-mCherry plasmids were electroporated into 4 dpf larvae and labeled electroporated M-cells with mCherry fluorescent protein. Axotomy and imaging were performed at 6 and 8 dpf, respectively (Figure 4D). We found that in comparison with axon regeneration in the control, axonal regeneration was retarded after DUSP2 overexpression (P = 0.0181; Figure 4E and F). Taken together, these results revealed that DUSP2 overexpression leads to reduced axonal regeneration.