Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (1): 24-30.doi: 10.3969/j.issn.1673-5374.2013.01.003

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A Disintegrin and Metalloprotease 10 in neuronal maturation and gliogenesis during cortex development

Zhixing Ma, Qingyu Li, Zhengyu Zhang, Yufang Zheng   

  1. School of Life Sciences, Fudan University, Shanghai 200433, China
  • Received:2012-07-16 Revised:2012-10-24 Online:2013-01-05 Published:2013-01-05
  • Contact: Yufang Zheng, Ph.D., Associate professor, School of Life Sciences, Fudan University, Shanghai 200433, China,zhengyf@fudan.edu.cn.
  • About author:Zhixing Ma★, Master.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 30800322; Shanghai Pujiang Program, No. 08PJ1401300; Shanghai Leading Academic Discipline Project, No. B111; Ministry of Education Research Fund for New Teachers in Doctoral Program of Higher Educational Institutes, No. 200802461050; National Basic Research Program of China (973 Program), No. 2011CB503703; Ministry of Education Start Fund to Returned Overseas Scholars; and ZhuoXue Program of Fudan University.

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Abstract:

The multiple-layer structure of the cerebral cortex is important for its functions. Such a structure is generated based on the proliferation and differentiation of neural stem/progenitor cells. Notch functions as a molecular switch for neural stem/progenitor cell fate during cortex development but the mechanism remains unclear. Biochemical and cellular studies showed that Notch receptor activation induces several proteases to release the Notch intracellular domain (NICD). A Disintegrin and Metalloprotease 10 (ADAM10) might be a physiological rate-limiting S2 enzyme for Notch activation. Nestin-driven conditional ADAM10 knockout in mouse cortex showed that ADAM10 is critical for maintenance of the neural stem cell population during early embryonic cortex development. However, the expression pattern and function of ADAM10 during later cerebral cortex development remains poorly understood. We performed in situ hybridization for ADAM10 mRNA and immunofluorescent analysis to determine the expression of ADAM10 and NICD in mouse cortex from embryonic day 9 (E14.5) to postnatal day 1 (P1). ADAM10 and NICD were highly co-localized in the cortex of E16.5 to P1 mice. Comparisons of expression patterns of ADAM10 with Nestin (neural stem cell marker), Tuj1 (mature neuron marker), and S100β (glia marker) showed that ADAM10 expression highly matched that of S100β and partially matched that of Tuj1 at later embryonic to early postnatal cortex developmental stages. Such expression patterns indicated that ADAM10-Notch signaling might have a critical function in neuronal maturation and gliogenesis during cortex development.

Key words: neural regeneration, neurogenesis, ADAM10, A Disintegrin and Metalloprotease, Notch, Notch intracellular domain, Tuj1, S100β, Nestin, cerebral cortex, development, neuronal maturation, glial cell, grants-supported paper, photographs-containing paper, neuroregeneration