Three-dimensional structure of axonal mitochondria reflects the age of drosophila

doi:10.3969/j.issn.1673-5374.2013.07.005

Abstract

Abstract:

This study aimed to reconstruct a three-dimensional map of axonal mitochondria using Fiji and Neurolucida software, and to observe directly the morphology and distribution of mitochondria in axons of motor neurons in dorsal longitudinal flight muscles of drosophila aged 5 days and 20 days, using electron microscopy. Results indicated that there was no difference in the total area and volume of mitochondria between 5-day-old drosophila and 20-day-old drosophila in all sections, but the ratio of mitochondrial total areas to axon total areas, as well as mitochondrial density of 20-day-old drosophila, was lower than that of 5-day-old drosophila. The number of mitochondria, whose volume was less than 1 000 000 μm³, and between 1 000 000 μm³ and 10 000 000 μm³, was higher in 20-day-old drosophila than that in 5-day-old drosophila. The number of mitochondria with a volume between 1 000 000 μm³ and 100 000 000 μm³ was apparently higher than those with a volume less than 1 000 000 μm³or larger than 100 000 000 μm³. In addition, the number of mitochondria with a volume more than 100 000 000 μm³ was small; however, the volume was nearly 70% of the total volume in both 5-day-old and 20-day-old drosophila. In contrast, the number of mitochondria with a volume between 1 000 000 μm³ and 10 000 000 μm³ was large, but the volume was less than 30% of the total volume. These experimental findings suggest that changes in mitochondrial morphology and number in motor neurons from the dorsal longitudinal muscle of drosophila are present during different ages.

Key words: neural regeneration, neurodegenerative diseases, neurogenesis, mitochondrion, drosophila, axon, three-dimensional model, electron microscopy, age-related neurodegenerative diseases, mitochondrial morphology, cell microstructure, photographs-containing paper, neuroregeneration

Honglian Zhu, Xiaojiang Sun. Three-dimensional structure of axonal mitochondria reflects the age of drosophila[J]. Neural Regeneration Research, 2013, 8(7): 616-621.

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