Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (2): 278-284.doi: 10.4103/1673-5374.177736

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Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury

Ming-san Miao, Lin Guo, Rui-qi Li, Xiao Ma   

  1. Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
  • Received:2015-07-08 Online:2016-02-15 Published:2016-02-15
  • Contact: Ming-san Miao, M.D.,miaomingsan@126.com.
  • Supported by:

    This study was supported by a grant from the State “Major New Drug Creation” Science and Technology Major Project in China, No. 2009ZX09103-324; a grant from the Henan Provincial Science and Technology Innovation Team in University in China, No. 2012IRTSTHN011.

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Abstract:

Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the recombinant human granulocyte colony stimulating factor-mobilized bone marrow stem cell transplantation on cerebral ischemia/reperfusion injury. Rat ischemia models were administered 0.3, 0.15 and 0.075 g/kg Radix Ilicis Pubescentis total flavonoids from 3 days before modeling to 2 days after injury. Results showed that Radix Ilicis Pubescentis total flavonoids
could reduce pathological injury in rats with cerebral ischemia/reperfusion injury. The number of Nissl bodies increased, Bax protein expression decreased, Bcl-2 protein expression increased and the number of CD34-positive cells increased. Therefore, Radix Ilicis Pubescentis total flavonoids can improve the bone marrow stem cell mobilization effect, enhance the anti-apoptotic ability of nerve cells, and have a neuroprotective
effect on cerebral ischemia/reperfusion injury in rats.

Key words: nerve regeneration, Radix Ilicis Pubescentis total flavonoids, bone marrow stem cells, mobilization, cerebral ischemia/reperfusion, Nissl bodies, Bax, Bcl-2, CD34, neurons, neural regeneration