Neural Regeneration Research ›› 2020, Vol. 15 ›› Issue (2): 315-323.doi: 10.4103/1673-5374.265556

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Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling

Shuai Wei1, 2, 3, Xue-Zhen Liang2, 4, Qian Hu1, Wei-Shan Wang1, Wen-Jing Xu2, Xiao-Qing Cheng2, 3, Jiang Peng2, 3, Quan-Yi Guo2, Shu-Yun Liu2, Wen Jiang1, 2, 3, Xiao Ding1, 2, 3, Gong-Hai Han2, 3, 5, Ping Liu2, 3, 6, Chen-Hui Shi1, Yu Wang2, 3#br#   

  1. 1 The First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China
    2 Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China
    3 Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
    4 The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China
    5 Kunming Medical University, Kunming, Yunnan Province, China
    6 Shanxi Medical University, Taiyuan, Shanxi Province, China
  • Online:2020-02-15 Published:2020-05-25
  • Contact: Yu Wang, MD,wangwangdian628@126.com; Chen-Hui Shi,1512905535@qq.com.
  • Supported by:
    This study was supported by National Key Research & Development Program of China, No. 2016YFC11011601, 2017YFA0104701, the Youth Cultivation Project of Military Medical Science, China, No. 15QNP091 (to YW), and People’s Liberation Army Youth Training Project for Medical Science of China, No. 16QNP144 (to YW).

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Abstract: Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between sensory and motor nerves in Wallerian degeneration and the characteristics of peripheral nerve regeneration. The study was approved by the Ethics Committee of the Chinese PLA General Hospital, China (approval No. 2016-x9-07) in September 2016.

Key words: gene ontology, Kyoto Encyclopedia of Genes and Genomes, isobaric tags for relative and absolute quantitation, motor nerve, proteomics, sensory nerve, spinal nerve, Wallerian degeneration