Neural Regeneration Research ›› 2024, Vol. 19 ›› Issue (2): 409-415.doi: 10.4103/1673-5374.375344

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Activation of endogenous neurogenesis and angiogenesis by basic fibroblast growth factor-chitosan gel in an adult rat model of ischemic stroke

Hongmei Duan1, #, Shulun Li1, #, Peng Hao1, Fei Hao2, Wen Zhao1, Yudan Gao1, Hui Qiao3, Yiming Gu4, Yang Lv3, Xinjie Bao5, Kin Chiu6, Kwok-Fai So7, 8, 9, 10, 11, *, Zhaoyang Yang1, *, Xiaoguang Li1, 12, *   

  1. 1Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China; 2Beijing Key Laboratory for Biomaterials and Neural Regeneration, School of Engineering Medicine, Beihang University, Beijing, China; 3Department of Epidemiology and Statistics, School of Public Health and Management, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 4Department of Physical Education, Capital University of Economics and Businessm, Beijing, China; 5Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; 6Department of Psychology, State Key Lab of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong Special Administration Region, China; 7Guangdong-Hongkong-Macau Institute of CNS Regeneration, Ministry of Education CNS Regeneration Collaborative Joint Laboratory, Jinan University, Guangzhou, Guangdong Province, China; 8Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, Guangdong Province, China; 9Department of Ophthalmology and State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong Special Administration Region, China; 10Center for Brain Science and Brain-Inspired Intelligence, Guangdong-Hong Kong-Macao Greater Bay Area, Guangzhou, Guangdong Province, China; 11Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China; 12Department of Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, China
  • Online:2024-02-15 Published:2023-08-30
  • Contact: Kwok-Fai So, PhD, hrmaskf@hku.hk; Zhaoyang Yang, PhD, wack_lily@163.com; Xiaoguang Li, PhD, lxgchina@sina.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, Nos. 81941011 (to XL), 31771053 (to HD), 31730030 (to XL), 31971279 (to ZY), 31900749 (to PH), 31650001(to XL), 31320103903 (to XL), 31670988 (to ZY); and the Natural Science Foundation of Beijing, Nos. 7222004 (to HD); and a grant from Ministry of Science and Technology of China, Nos. 2017YFC1104002 (to ZY), 2017YFC1104001 (to XL); a grant from Beihang University, No. JKF-YG-22-B001 (to FH).

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Abstract: Attempts have been made to use cell transplantation and biomaterials to promote cell proliferation, differentiation, migration, and survival, as well as angiogenesis, in the context of brain injury. However, whether bioactive materials can repair the damage caused by ischemic stroke by activating endogenous neurogenesis and angiogenesis is still unknown. In this study, we applied chitosan gel loaded with basic fibroblast growth factor to the stroke cavity 7 days after ischemic stroke in rats. The gel slowly released basic fibroblast growth factor, which improved the local microenvironment, activated endogenous neural stem/progenitor cells, and recruited these cells to migrate toward the penumbra and stroke cavity and subsequently differentiate into neurons, while enhancing angiogenesis in the penumbra and stroke cavity and ultimately leading to partial functional recovery. This study revealed the mechanism by which bioactive materials repair ischemic strokes, thus providing a new strategy for the clinical application of bioactive materials in the treatment of ischemic stroke.

Key words: adult endogenous neurogenesis, angiogenesis, basic fibroblast growth factor-chitosan gel, chitosan, functional recovery, ischemic stroke, neural stem cell, newborn neuron