Abstract: Since the pioneering work by Broca and Wernicke in the 19th century, who examined individuals with brain lesions to associate them with specific behaviors, it was evident that behaviors are complex and cannot be fully attributable to specific brain areas alone. Instead, they involve connectivity among brain areas, whether close or distant. At that time, this approach was considered the optimal way to dissect brain circuitry and function. These pioneering efforts opened the field to explore the necessity or sufficiency of brain areas in controlling behavior and hence dissecting brain function. However, the connectivity of the brain and the mechanisms through which various brain regions regulate specific behaviors, either individually or collaboratively, remain largely elusive. Utilizing animal models, researchers have endeavored to unravel the necessity or sufficiency of specific brain areas in influencing behavior; however, no clear associations have been firmly established.