Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (12): 917-923.

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Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

Fengguo Zhai1, Xiuping Zhang1, Yue Guan2, Xudong Yang1, Yang Li3, Gaochen Song4, Lixin Guan1   

  1. 1 Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
    2 Department of Endocrinology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
    3 Department of Thoracic Surgery, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
    4 Department of Biochemistry, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
  • Received:2011-11-01 Revised:2012-02-24 Online:2012-04-25 Published:2012-04-25
  • Contact: Guan, M.D., Professor, Department of Pharmacology, Mudanjiang Medical University Mudanjiang 157011, Heilongjiang Province, China; Gaochen Song, M.D., Professor, Department of Biochemistry, Mudanjiang MedicaI University, Mudanjiang 157011, Heilongjiang Province, China glx1960@yahoo.com.cn; songgaochen@yahoo.com.cn
  • About author:Fengguo Zhai☆, M.D., Associate professor, Department of Pharmacology, Mudanjiang MedicaI University, Mudanjiang 157011, Heilongjiang Province, China

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Abstract:

Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.

Key words: focal cerebral ischemia, microRNA, apoptosis, bcl-2, caspase-3, neural regeneration