Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (17): 1293-1298.

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Perlecan and synaptophysin changes in denervated skeletal muscle

Kai Ma1, 2, Zhifeng Huang3, Jianfeng Ma2, Longquan Shao4, Huiming Wang1, Yanliang Wang2   

  1. 1  Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China
    2  Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 3  25027, Zhejiang Province, China
    3  School of Pharmacy, Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
    4  Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2012-01-19 Revised:2012-04-23 Online:2012-06-15 Published:2012-06-15
  • Contact: Huiming Wang, Professor, Doctoral supervisor, De-partment of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China; Yanliang Wang, Associate chief physician, Master’s supervisor, De-partment of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China wangmysm@yahoo.com.cn wangyanliang@hotmail.com
  • About author:Kai Ma☆, M.D., Attending physician, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China

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Abstract:

The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix com-ponent, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle dege-nerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synap-tophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

Key words: perlecan, synaptophysin, extracellular matrix, acetylcholine esterase, neuromuscular junction, denervated, skeletal muscle, sciatic nerve, neural regeneration