Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (9): 659-663.

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Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer’s disease Effects and a comparison with donepezil

Ren’an Qin1, Desheng Zhou2, Jiajun Wang2, Hua Hu2, Yang Yang2, Xiaoxuan Yao1, Xiaopeng Sun2   

  1. 1  Modern Chinese Medicine Research Institute of Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd., Guangzhou 510515, Guangdong Province, China
    2  Department of Neurology, First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
  • Received:2011-11-24 Revised:2012-01-17 Online:2012-03-25 Published:2012-03-25
  • Contact: Desheng Zhou, M.D., Professor, Department of Neurology, First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China zds1101@tom.com
  • About author:Ren’an Qin☆, M.D., Associate chief pharmacist, Modern Chinese Medicine Research Institute of Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd., Guangzhou 510515, Guangdong Province, China

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Abstract:

After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer’s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer’s disease, with similar effects to donepezil.

Key words: amyloid protein precursor, Alzheimer’s disease, transgenic cell model, compound Danshen tablets, Chinese medicine, neural regeneration