Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (33): 3124-3130.doi: 10.3969/j.issn.1673-5374.2013.33.006

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Substance P and calcitonin gene-related peptide expression in dorsal root ganglia in sciatic nerve injury rats

Changma Fu1, Zongsheng Yin1, Defu Yu1, Zuhua Yang2   

  1. 1 Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
    2 Third Clinical College, Anhui Medical University, Hefei 230022, Anhui Province, China
  • Received:2013-04-30 Revised:2013-06-13 Online:2013-11-25 Published:2013-11-25
  • Contact: Zongsheng Yin, M.D., Chief physician, Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China, yinzongsheng@sina.com.
  • About author:Changma Fu, M.D., Attending physician.
  • Supported by:

    This study was supported by the Major Program of Science and Technology of Ministry of Education, No. 207049.

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Abstract:

The neuropeptides, substance P and calcitonin gene-related peptide, have been shown to be involved in pain transmission and repair of sciatic nerve injury. A model of sciatic nerve defect was prepared by dissecting the sciatic nerve at the middle, left femur in female Sprague Dawley rats. The two ends of the nerve were encased in a silica gel tube. L5 dorsal root ganglia were harvested 7, 14 and 28 days post sciatic nerve injury for immunohistochemical staining. Results showed that substance P and cal-citonin gene-related peptide expression increased significantly in dorsal root ganglion of rats with sci-atic nerve injury. This increase peaked at 7 days, declined at 14 days, and reduced to normal levels by 28 days post injury. The findings indicate that the neuropeptides, substance P and calcitonin gene- related peptide, mainly increased in the early stages after sciatic nerve injury.

Key words: neural regeneration, peripheral nerve injury, sciatic nerve, dorsal root ganglion, spinal cord, neuropeptides, calcitonin gene-related peptide, substance P, pain, neuroprotection, grants- supported paper, neuroregeneration