Abstract: Alzheimer’s disease (AD) is a neurodegenerative disease that is characterized by an age-dependent progressive decline of memory, impairment of cognitive functions and changes in personality and behavior. Despite the improvement in understanding of the mechanisms underlying the disease, AD remains an incurable complex disorder with multifaceted pathophysiology to date. Apolipoprotein E (ApoE) is the main cholesterol carrier in the brain that supports lipid transport between brain cells. The individuals carrying the APOE4 allele are known to be at increased risk of developing AD compared with those carrying the more common APOE3 allele. Many researches have been undertaken to understand the role of APOE4 on brain cells and in AD (Shi et al., 2017). Despite the APOE allele being identified as an important genetic risk factor for cardiovascular disease and formation of blood vessels, there is comparatively less research focused on the blood-brain barrier functions in AD. The complex nature of the blood-brain barrier (BBB) and species differences hindered the development in this field. Recent advancement to induced pluripotent stem cell (iPSC) technologies provides an ideal platform to fine-tune BBB models and the possibilities to develop isogeneic models now allow us to improve our knowledge of the BBB and to model more disease relevant models.