Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (23): 2074-2080.doi: 10.4103/1673-5374.147934

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Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Feng Zhou 1, Liang Wang 1, 2, Panpan Liu 1, Weiwei Hu 1, Xiangdong Zhu 1, Hong Shen 1, Yuanyuan Yao 3   

  1. 1 Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
    2 Medical College, Ningbo University, Ningbo, Zhejiang Province, China
    3 Department of Anesthesiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
  • Received:2014-08-18 Online:2014-12-10 Published:2014-12-10
  • Contact: Yuanyuan Yao, M.D., Department of Anesthesiology, Second Affiliated Hospital,School of Medicine, Zhejiang University,Hangzhou 310009, Zhejiang Province,China, yuanyuan58@126.com.
  • Supported by:

    This study was supported by the Chinese Traditional Medical Science Foundation of Zhejiang Province in China, No. 2010ZA072; the Health Bureau Foundation of Zhejiang Province in China, No. 2012ZDA023; and the Qianjiang Project of Zhejiang Science and Technology Bureau in China, No. 2010 R10073.

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Abstract:

Puerarin, a traditional Chinese medicine, exerts a powerful neuroprotective effect in cerebral ischemia/reperfusion injury, but its mechanism is unknown. Here, we established rat models of middle cerebral artery ischemia/reperfusion injury using the suture method. Puerarin (100 mg/kg) was administered intraperitoneally 30 minutes before middle cerebral artery occlusion and 8 hours after reperfusion. Twenty-four hours after reperfusion, we found that puerarin significantly improved neurological deficit, reduced infarct size and brain water content, and notably diminished the expression of Toll-like receptor-4, myeloid differentiation factor 88, nuclear factor kappa B and tumor necrosis factor-α in the ischemic region. These data indicate that puerarin exerts an anti-inflammatory protective effect on brain tissue with ischemia/reperfusion damage by downregulating the expression of multiple inflammatory factors.

Key words: nerve regeneration, brain injury, puerarin, cerebral ischemia, reperfusion injury, rats, inflammatory reaction, Toll-like receptor-4, nuclear factor kappa B, myeloid differentiation factor 88, tumor necrosis factor-α, middle cerebral artery occlusion, neural regeneration