Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (11): 1773-1778.doi: 10.4103/1673-5374.194747

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NADPH oxidase 2 does not contribute to early reperfusion-associated reactive oxygen species generation following transient focal cerebral ischemia

Yuan Zhang1, 2, 3, Ting Wang1, 2, 4, Ke Yang1, 2, 4, Ji Xu1, 2, Jian-ming Wu5, Wen-lan Liu1, 2, *   

  1. 1 Central Laboratory, Shenzhen Second People’s Hospital, First Afliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China 2 Shenzhen Key Laboratory of Neurosurgery, Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China 3 Department of Pathophysiology, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region, China 4 Graduate School of Guangzhou Medical University, Guangzhou, Guangdong Province, China 5 Department of Neurosurgery, Shenzhen Second People’s Hospital, First Afliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China
  • Received:2016-09-20 Online:2016-11-30 Published:2016-11-30
  • Contact: Wen-lan Liu, M.D., Ph.D., wlliu@szu.edu.cn.
  • Supported by:

    The work was financially supported by grants from Shenzhen Science and Technology Innovation Commission of China, No. JCYJ20150330102401097, KQCX20140521101427034, JCYJ20140414170821291, and China Postdoctoral Science Foundation, No. 2015M572388.

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Abstract:

Excess production of reactive oxygen species (ROS) critically contributes to occurrence of reperfusion injury, the paradoxical response of ischemic brain tissue to restoration of cerebral blood ?ow. However, the enzymatic sources of ROS generation remain to be unclear. Tis study examined Nox2-containing NADPH oxidase (Nox2) expression and its activity in ischemic brain tissue following post-ischemic reperfusion to clarify the mechanism of enzymatic reaction of ROS. Male Sprague-Dawley rats were subjected to 90-minute middle cerebral artery occlusion, followed by 3 or 22.5 hours of reperfusion. Quantitative reverse transcriptase PCR and western blot assay were performed to measure mRNA and protein expression of Nox2. Lucigenin ?uorescence assays were performed to assess Nox activity. Our data showed that Nox2 mRNA and protein expression levels were signifcantly increased (3.7-fold for mRNA and 3.6-fold for protein) in ischemic brain tissue at 22.5 hours but not at 3 hours following post-ischemic reperfusion. Similar results were obtained for the changes of NADPH oxidase activity in ischemic cerebral tissue at the two reperfusion time points. Our results suggest that Nox2 may not contribute to the early burst of reperfusion-related ROS generation, but is rather an important source of ROS generation during prolonged reperfusion.

Key words: nerve regeneration, NADPH oxidase, cerebral ischemia, Nox family, reactive oxygen species, reperfusion, central nervous system, stroke, blood ?ow, neural regeneration