Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (8): 601-605.

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Establishment of primary cultures of craniopharyngioma cells

Hao Liu1, Liang Liu2, Zhiyong Liu1, Qiang Li1, Chao You1, Jianguo Xu1   

  1. 1 Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
    2 Department of Neurosurgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Received:2011-12-10 Revised:2012-02-06 Online:2012-03-15 Published:2012-03-15
  • Contact: Jianguo Xu, M.D., Associate professor, Master’s supervisor, Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China Jianguo_1229@sina.com
  • About author:Hao Liu★, Master, Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China Hao Liu and Liang Liu contributed equally to this work.

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Abstract:

Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 passages, confirming that all cultured cells were squamous epithelial cells. The doubling time of craniopharyngioma cells was 3 days, as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In this study, craniopharyngioma cells cultured in vitro were established; however, establishment of immortalized craniopharyngioma cell lines requires further research.

Key words: craniopharyngioma, cytokeratin-7, primary culture, culture in vitro, cell line