Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (3): 233-243.doi: 10.3969/j.issn.1673-5374.2013.03.005

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Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles

Junming Tan1, Jiangang Shi2, Guodong Shi2, Yanling Liu3, Xiaohong Liu3, Chaoyang Wang1, Dechun Chen1, Shunming Xing1, Lianbing Shen1, Lianshun Jia2, Xiaojian Ye2, Hailong He2, Jiashun Li2   

  1. 1 Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China
    2 Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
    3 Department of Pathologic Laboratory of Chest Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2012-07-28 Revised:2012-10-10 Online:2013-01-25 Published:2013-01-25
  • Contact: Jiangang Shi, M.D., Associate professor, Chief physician, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China, tanjunm@sina.com.
  • About author:Junming Tan☆, M.D., Associate chief physician, Associate professor, Master’s supervisor.
  • Supported by:

    This study was financially supported by grants from the Medical Scientific Fund and Intensive Research of Nanjing Military Area Command of Chinese PLA, No. Nan 2007-13 and Nan 08Z003; and the Medical Scientific Fund and Research of Chinese PLA during the 12th Five-Year Plan Period, No. CWS11J260.

Abstract:

This study established a dog model of acute multiple cauda equina constriction by experimental constriction injury (48 hours) of the lumbosacral central processes in dorsal root ganglia neurons. The repair effect of intrathecal injection of brain-derived neurotrophic factor with 15 mg encapsulated biodegradable poly(lactide-co-glycolide) nanoparticles on this injury was then analyzed. Dorsal root ganglion cells (L7) of all experimental dogs were analyzed using hematoxylin-eosin staining and immunohistochemistry at 1, 2 and 4 weeks following model induction. Intrathecal injection of brain-derived neurotrophic factor can relieve degeneration and inflammation, and elevate the expression of brain-derived neurotrophic factor in sensory neurons of compressed dorsal root ganglion. Simultaneously, intrathecal injection of brain-derived neurotrophic factor obviously improved neurological function in the dog model of acute multiple cauda equina constriction. Results verified that sustained intraspinal delivery of brain-derived neurotrophic factor encapsulated in biodegradable nanoparticles promoted the repair of histomorphology and function of neurons within the dorsal root ganglia in dogs with acute and severe cauda equina syndrome.

Key words: neural regeneration, peripheral nerve injury, cauda equina syndrome, dorsal root ganglion, brain-derived neurotrophic factor, multiple cauda equina constrictions, neurotrophic factors, neural protection, grants-supported paper, photographs-containing paper, neuroregeneration