中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (25): 1925-1930.doi: 10.3969/j.issn.1673-5374.2012.25.001

• 原著:脑损伤修复保护与再生 •    下一篇

体外模拟脑内微环境中孕酮促进人脐带间充质干细胞向神经元样细胞的神经分化

  

  • 收稿日期:2011-03-06 修回日期:2011-05-04 出版日期:2012-09-05 发布日期:2012-09-05

Progesterone promotes neuronal differentiation of human umbilical cord mesenchymal stem cells in culture conditions that mimic the brain microenvironment

Xianying Wang1, 2, Honghai Wu3, Gai Xue3, Yanning Hou1, 3   

  1. 1 Hebei Medical University, Shijiazhuang 050017, Hebei Province, China 
    2 The Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China 
    3 Bethune International Peace Hospital of Chinese PLA, Shijiazhuang 050082, Hebei Province, China
  • Received:2011-03-06 Revised:2011-05-04 Online:2012-09-05 Published:2012-09-05
  • Contact: Yanning Hou, Master, Master’s supervisor, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China; Bethune International Peace Hospital of Chinese PLA, Shijiazhuang 050082, Hebei Province, China
  • About author:Xianying Wang★, Studying for master’s degree, Asso-ciate chief pharmacist, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China; The Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China
  • Supported by:

    This work was supported by the Military Medical Research Program during the 12th Five-Year Plan Period, No. BWS11J002.

PDF

872

Abstract:

In this study, human umbilical cord mesenchymal stem cells from full-term neonates born by vaginal delivery were cultured in medium containing 150 mg/mL of brain tissue extracts from Sprague-Dawley rats (to mimic the brain microenvironment). Immunocytochemical analysis demonstrated that the cells differentiated into neuron-like cells. To evaluate the effects of progesterone as a neurosteroid on the neuronal differentiation of human umbilical cord mesenchymal stem cells, we cultured the cells in medium containing progesterone (0.1, 1, 10 μM) in addition to brain tissue extracts. Reverse transcription-PCR and flow cytometric analysis of neuron specific enolase-positive cells revealed that the percentages of these cells increased significantly following progesterone treatment, with the optimal progesterone concentration for neuron-like differentiation being 1 μM. These results suggest that progesterone can enhance the neuronal differentiation of human umbilical cord mesenchymal stem cells in culture medium containing brain tissue extracts to mimic the brain microenvironment.

Key words: progesterone, mesenchymal stem cells, neuron, differentiation, brain tissue extracts, neural regeneration