中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (25): 1960-1966.doi: 10.3969/j.issn.1673-5374.2012.25.006

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

颅脑损伤大鼠脑红蛋白的表达

  

  • 收稿日期:2012-05-11 修回日期:2012-07-10 出版日期:2012-09-05 发布日期:2012-09-05

Neuroglobin expression in rats after traumatic brain injury

Xin Lin1, Min Li2, Aijia Shang3, Yazhuo Hu4, Xiao Yang5, Ling Ye4, Suyan Bian6, Zhongfeng Wang7, Dingbiao Zhou3   

  1. 1 Department of Surgery, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China
    2 Department of Traumatic-Aesthetic Surgery, Huangsi Aesthetic Surgery Hospital, Beijing 100120, China
    3 Department of Neurosurgery, Division of Surgery, General Hospital of Chinese PLA, Beijing 100853, China
    4 Institute of Geriatrics, General Hospital of Chinese PLA, Beijing 100853, China
    5 Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
    6 Department of Cardiology, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China
    7 Institute of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
  • Received:2012-05-11 Revised:2012-07-10 Online:2012-09-05 Published:2012-09-05
  • Contact: 1 Department of Surgery, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China 2 Department of Traumatic-Aesthetic Surgery, Huangsi Aesthetic Surgery Hospital, Beijing 100120, China 3 Department of Neurosurgery, Division of Surgery, General Hospital of Chinese PLA, Beijing 100853, China 4 Institute of Geriatrics, General Hospital of Chinese PLA, Beijing 100853, China 5 Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China 6 Department of Cardiology, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China 7 Institute of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
  • About author:Xin Lin☆, M.D., Attending physician, Department of Surgery, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China

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Abstract:

In this study, we used a rat model of severe closed traumatic brain injury to explore the relationship between neuroglobin, brain injury and neuronal apoptosis. Real-time PCR showed that neuroglobin mRNA expression rapidly increased in the rat cerebral cortex, and peaked at 30 minutes and
48 hours following traumatic brain injury. Immunohistochemical staining demonstrated that neu-roglobin expression increased and remained high 2 hours to 5 days following injury. The rate of increase in the apoptosis-related Bax/Bcl-2 ratio greatly decreased between 30 minutes and 1 hour as well as between 48 and 72 hours post injury. Expression of neuroglobin and the anti-apoptotic factor Bcl-2 greatly increased, while that of the proapoptotic factor decreased, in the cerebral cortex post severe closed traumatic brain injury. It suggests that neuroglobin might protect neurons from apoptosis after traumatic injury by regulating Bax/Bcl-2 pathway.

Key words: neuroglobin, traumatic brain injury, neuron, apoptosis, cerebral cortex, Bax, Bcl-2, neural regeneration