中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (25): 1967-1973.doi: 10.3969/j.issn.1673-5374.2012.25.007

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

丰富环境提高产前应激子代大鼠认知能力及海马生长相关蛋白43的表达

  

  • 收稿日期:2012-05-11 修回日期:2012-07-10 出版日期:2012-09-05 发布日期:2012-09-05

Enriched environment upregulates growth- associated protein 43 expression in the hippocampus and enhances cognitive abilities in prenatally stressed rat offspring

Zhengyu Zhang1, Hua Zhang2, Baoling Du1, Zhiqiang Chen3   

  1. 1 Department of Histology and Embryology, Guangzhou Medical University, Guangzhou 510185, Guangdong Province, China
    2 Department of Stomatology, First Affiliated Hospital, Jinan University, Guangzhou 510630, Guangdong Province, China
    3 Department of Orthopedics Surgery, Liaocheng People’s Hospital, Liaocheng 252000, Shandong Province, China
  • Received:2012-05-11 Revised:2012-07-10 Online:2012-09-05 Published:2012-09-05
  • Contact: Zhengyu Zhang, Department of Histology and Embryology, Guangzhou Medical University, Guangzhou 510185, Guangdong Province, China; Zhiqiang Chen, Master, Attending physician, Department of Orthopedics Surgery, Liaocheng People’s Hospital, Liaocheng 252000, Shandong Province, China 50465033@qq.com; 18044807@qq.com
  • About author:Zhengyu Zhang★, Master, Lecturer, Department of Histology and Embryology, Guangzhou Medical University, Guangzhou 510185, Guangdong Province, China Zhengyu Zhang and Hua Zhang contributed equally to this article.

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Abstract:

In our previous study, we reported that prenatal restraint stress could induce cognitive deficits, which correlated with a change in expression of growth-associated protein 43 in the hippocampus. In this study, we investigated the effects of enriched environment on cognitive abilities in prenatally stressed rat offspring, as well as the underlying mechanisms. Reverse transcription-PCR and western blot assay results revealed that growth-associated protein 43 mRNA and protein levels were upregulated on postnatal day 15 in the prenatal restraint stress group. Growth-associated protein 43 expression was significantly lower in the prenatal restraint stress group compared with the negative control and prenatal restraint stress plus enriched environment groups on postnatal days 30 and 50. Morris water maze test demonstrated that cognitive abilities were noticeably increased in rats from the prenatal restraint stress plus enriched environment group on postnatal day 50. These results indicate that enriched environment can improve the spatial learning and memory ability of prenatally stressed offspring by upregulating growth-associated protein 43 expression.

Key words: prenatal restraint stress, growth-associated protein 43, hippocampus, Morris water maze, enriched environment, cognition, neural regeneration