中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (5): 453-455.doi: 10.4103/1673-5374.130052

• 观点:脊髓损伤修复保护与再生 •    下一篇

啮齿动物脊髓损伤模型的技术评论

  

  • 收稿日期:2014-02-18 出版日期:2014-03-12 发布日期:2014-03-12

Technical comments on rodent spinal cord injuries models

Zoe Zhang1, Yi Ping Zhang2, Lisa B. E. Shields2, Christopher B. Shields2     

  1. 1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN 55455, USA
    2 Norton Neuroscience Institute, Norton Healthcare, Louisville, KY 40202, USA  
  • Received:2014-02-18 Online:2014-03-12 Published:2014-03-12

摘要:

脊髓损伤机制及潜在的治疗方法研究都依赖于可靠的动物模型。以Christopher B. Shields博士为首的美国肯塔基州路易斯维尔的诺顿神经科学研究所,30年来一直致力于脊髓损伤基础与临床研究。Shields博士和他的团队专注于建立小鼠/大鼠中可重复的、可靠的和精确的挫裂伤脊髓损伤模型的方法。他们的实验室已经开发出了新的方法来创建椎体稳定性,提供了精确的撕裂伤和挫伤设备,并描述了几种监视脊髓损伤严重程度和进展的神经生理学的方法。

Abstract:

Animal models are required to investigate mechanisms of spinal cord injuries (SCI) and to gain insight into potential treatments. This article describes several experimental animal models that mimic the clinical picture of SCI. Advantages and disadvantages of each method are compared.  Common technical errors in creating experimental SCI are reviewed including poor vertebral stabilization and variability in segmental location. The creation of reliable models of SCI is essential to avoid erroneous outcomes in the laboratory. Prevention of such errors in experimental rodent models may avoid costly and time-consuming clinical trials from being performed.