中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (10): 1003-1013.doi: 10.4103/1673-5374.133159

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

运动神经元样细胞移植联合胶质细胞源性神经营养因子修复脊髓挫伤

  

  • 收稿日期:2014-03-18 出版日期:2014-05-26 发布日期:2014-05-26

Intraspinal transplantation of motoneuron-like cell combined with delivery of polymer-based glial cell line-derived neurotrophic factor  for repair of spinal cord contusion injury

Alireza Abdanipour, Taki Tiraihi, Taher Taheri   

  1. Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, Iran
  • Received:2014-03-18 Online:2014-05-26 Published:2014-05-26
  • Contact: Taki Tiraihi, Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Jalal El Ahmed express way, P.O. Box 14155-4838, Tehran, Iran, takialtr@modares.ac.ir; ttiraihi@gmail.com.
  • Supported by:

    The project was funded by Shefa Neurosciences Research Center at Khatam Al-Anbia Hospital, Tehran, Iran (Grant # 86-N-105).

摘要:

为评估胶质细胞源性神经营养因子联合脂肪干细胞转分化的运动神经元样细胞对大鼠脊髓挫伤的影响,实验于大鼠脊髓挫伤后7d,将脂肪干细胞诱导分化的运动神经元样细胞注射于脊髓挫伤大鼠脊髓损伤中心和头尾部,将胶质细胞源性神经营养因子-明胶海绵复合体植入髓鞘。结果显示,运动神经元样细胞移植联合胶质细胞源性神经营养因子可减少挫伤脊髓空洞的形成,提高细胞移植部位的神经细胞密度;且对脊髓损伤大鼠运动功能恢复的促进作用优于单独应用胶质细胞源性神经营养因子、脂肪干细胞、运动神经元样细胞的作用。说明此种联合疗法是修复脊髓损伤有潜力的方法。

Abstract:

To evaluate the effects of glial cell line-derived neurotrophic factor transplantation combined with adipose-derived stem cells-transdifferentiated motoneuron delivery on spinal cord contusion injury, we developed rat models of spinal cord contusion injury, 7 days later, injected adipose-derived stem cells-transdifferentiated motoneurons into the epicenter, rostral and caudal regions of the impact site and simultaneously transplanted glial cell line-derived neurotrophic factor-gelfoam complex into the myelin sheath. Motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery reduced cavity formations and increased cell density in the transplantation site. The combined therapy exhibited superior promoting effects on recovery of motor function to transplantation of glial cell line-derived neurotrophic factor, adipose-derived stem cells or motoneurons alone. These findings suggest that motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery holds a great promise for repair of spinal cord injury. 

Key words: nerve regeneration, spinal cord injury, adipose-derived stem cells, glial cell line-derived neurotrophic factor, motoneurons, cell transplantation, neurotrophic factor, spinal cord contusion injury, neural regeneration