中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (12): 1195-1203.doi: 10.4103/1673-5374.135328

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

槲皮素可通过自噬途径减轻高糖对许旺细胞的损害

  

  • 收稿日期:2014-05-16 出版日期:2014-06-24 发布日期:2014-06-24

Quercetin alleviates high glucose-induced Schwann cell damage by autophagy

Ling Qu 1, Xiaochun Liang 1, Bei Gu 2, Wei Liu 1   

  1. 1 Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, China Academy of Medical Sciences, Beijing, China
    2 Cell Center, Institute of Basic Medical Science, Peking Union Medical College, China Academy of Medical Sciences, Beijing, China
  • Received:2014-05-16 Online:2014-06-24 Published:2014-06-24
  • Contact: Ling Qu, M.D., Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, China Academy of Medical Sciences, Beijing 100730, China, quling@pumch.cn.

摘要:

因有证据显示,槲皮素可拮抗高糖抑制的神经细胞增殖,由此实验假设槲皮素对糖尿病周围神经病变能产生保护作用。实验结果显示,高糖环境下,永生化大鼠RSC96细胞和原代培养大鼠许旺细胞自噬小体明显减少,增殖活力和自噬分子标志物Beclin1和 LC3表达明显下降;而槲皮素干预后,2种许旺细胞自噬能力和增殖活力明显提高。说明槲皮素能通过自噬途径减轻高糖对许旺细胞的损害。

Abstract:

Quercetin can reverse high glucose-induced inhibition of neural cell proliferation, and therefore may have a neuroprotective effect in diabetic peripheral neuropathy. It is difficult to obtain primary Schwann cells and RSC96 cells could replace primary Schwann cells in studies of the role of autophagy in the mechanism underlying diabetic peripheral neuropathy. Here, we show that under high glucose conditions, there are fewer autophagosomes in immortalized rat RSC96 cells and primary rat Schwann cells than under control conditions, the proliferative activity of both cell types is significantly impaired, and the expression of Beclin-1 and LC3, the molecular markers for autophagy, is significantly lower. After intervention with quercetin, the autophagic and proliferative activity of both cell types is rescued. These results suggest that quercetin can alleviate high glucose-induced damage to Schwann cells by autophagy.

Key words: nerve regeneration, quercetin, diabetic peripheral neuropathy, high glucose, RSC96, primary Schwann cells, proliferation, ultrastructure, autophagy, Beclin-1, LC3, NSFC grant, neural regeneration