中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (13): 1283-1287.doi: 10.4103/1673-5374.137575

• 原著:退行性病与再生 • 上一篇    下一篇

益智健脑中药治疗阿尔茨海默病抑制神经元凋亡的机制

  

  • 收稿日期:2014-05-20 出版日期:2014-07-11 发布日期:2014-07-11
  • 基金资助:

    中南大学湘雅三医院“125人才工程”(07JJ5017).

Treating Alzheimer’s disease with Yizhijiannao granules by regulating expression of multiple proteins in temporal lobe

Hong Zhu 1, Liuyang Luo 2, Sihang Hu 1, Keli Dong 1, Guangcheng Li 1, Ting Zhang 1   

  1. 1 Department of Traditional Chinese Medicine, Third Xiangya Hospital of Central South University, Changsha, Hunan Province, China
    2 Department of Emergency, Bao-an District Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong Province, China
  • Received:2014-05-20 Online:2014-07-11 Published:2014-07-11
  • Contact: Guangcheng Li, Ph.D., Department of Traditional Chinese Medicine, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China, yellingu@163.com.
  • Supported by:

    This study was supported by Startup Fund for 125 Scholars of Third Xiangya Hospital; and the Natural Science Foundation of Hunan Province, No. 07JJ5017.

摘要:

课题组前期研究发现,复方中药益智健脑颗粒能够改善阿尔茨海默病患者的认知功能。鉴于此,实验希望进一步解释其作用机制。实验将益智健脑颗粒浓缩液灌胃干预存在学习记忆能力障碍的快速老化小鼠P8,设为治疗组灌胃8周,并将用双蒸水灌胃的快速老化小鼠P8设为对照组灌8周胃。双向凝胶电泳分离快速老化小鼠P8颞叶组织总蛋白,质谱技术得到相应肽指纹图谱进行蛋白分析,发现两组颞叶组织存在37个差异蛋白点,最终鉴定出10个差异蛋白质,High mobility group box 1,Dimethylarginine dimethylaminohydrolase -1,Neuroglobin,Hemoglobin beta adult major chain,Peroxiredoxin-6,Cofilin-1,Flotillin 1,Peptidylprollyl isomerase A,Voltage-dependent anion channel 2和Chaperonin containing TCP1, subunit 2,其功能分别涉及调节淀粉样β蛋白的生成、氧化应激、神经炎症、Tau蛋白磷酸化的调节、神经元凋亡的调节。结果显示,益智健脑颗粒能够调节快速老化小鼠P8颞叶组织多个蛋白的表达从而发挥神经元凋亡靶点治疗效应。

Abstract:

Yizhijiannao granules have been shown to improve cognitive function in Alzheimer’s disease patients. The present study sought to explore the mechanisms involved in the cognitive enhancing effects of Yizhijiannao granule. Senescence-accelerated mouse prone 8 mice with learning and memory disorders were intragastrically treated with Yizhijiannao granule for 8 weeks. Mice intragastrically treated with double distilled water for 8 weeks were considered as the control group. 2D gel electrophoresis was used to isolate total protein from the temporal lobe of senescence-accelerated mouse prone 8 mice, and differential protein spots were obtained by mass spectrometry. Thirty-seven differential protein spots were found in the temporal lobe area of both groups. Ten protein spots were identified: high mobility group box 1, dimethylarginine dimethylaminohydrolase-1, neuroglobin, hemoglobin beta adult major chain, peroxiredoxin-6, cofilin-1, flotillin 1, peptidylprolyl isomerase A, voltage-dependent anion channel-2 and chaperonin containing TCP1, and subunit 2. Among other functions, these proteins are separately involved in the regulation of amyloid beta production, oxidative stress, neuroinflammation, regulation of tau phosphorylation, and regulation of neuronal apoptosis. Our results revealed that Yizhijiannao granule can regulate the expression of various proteins in the temporal lobe of senescence-accelerated mouse prone 8 mice, and may be therapeutically beneficial for the treatment of Alzheimer’s disease.