中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 66-68.doi: 10.4103/1673-5374.165303

• 观点:神经损伤修复保护与再生 • 上一篇    下一篇

浓度游戏:2D和3D培养生物活性信号的差异效应

  

  • 收稿日期:2015-08-26 出版日期:2016-01-15 发布日期:2016-01-15
  • 基金资助:

    该研究是由TIRR基础项目,德克萨斯大学健康科学中心,休斯顿本特森脑卒中中心以及William Stamps Farishish基金神经外科部部分资助。

The concentration game: differential effects of bioactive signaling in 2D and 3D culture

Laura A. Smith Callahan   

  1. The Vivian L. Smith Department of Neurosurgery & Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center at Houston, Houston, TX, USA
  • Received:2015-08-26 Online:2016-01-15 Published:2016-01-15
  • Contact: Laura A. Smith Callahan, Ph.D.,Laura.A.SmithCallahan@uth.tmc.edu.
  • Supported by:

    Work was funded in part by Mission Connect, a program of TIRR foundation, the University of Texas Health Science Center at Houston Bentsen Stroke Center and Department of Neurosurgery William Stamps Farish Fund.

摘要:

人工细胞外基质或支架,一种改变非支持性环境并进而改善干细胞疗效,提高神经组织再生的方法。他们认为先进的支架可以更好地模仿细胞外基质的化学、物理和机制性能,用以促进细胞的存活、粘附、增殖和分化,进而改变受损的细胞外基质以减轻轴突、髓鞘和细胞成熟障碍等阻碍因素。在2D或3D培养条件下,浓度变化与细胞交互方式均可改变细胞存活、粘附。了解这些浓度变化的影响,能使我们更有效地操纵细胞外环境,如支架、药物、干细胞等,用来恢复创伤性脑和脊髓损伤后的神经功能。

Abstract:

Traumatic injuries to the central nervous system trigger a series of secondary biochemical and cellular responses that ultimately lead to cellular death and maintenance of an unsupportive extracellular matrix (ECM) for tissue regeneration. Artificial ECM or scaffolds represent a way to alter this unsupportive environment to improve the efficacy of stem cell therapies and enhance neural tissue regeneration. The inclusion of basic scaffolds with stem cell therapy treatments have shown increased efficacy in rodent models. More advanced scaffolds could better mimic the chemical, physical and mechanical properties of the ECM to promote survival, adhesion, proliferation and differentiation altering the injured ECM to mitigate the barriers to axon invasion, myelination and cellular maturation. Changes in IKVAV concentration have been implicated in altering cellular attachment and neural differentiation. In 2D culture, maximal neurite extension and neural gene expression occurred on hydrogels. While in 3D culture, neurite extension was delayed. The common thread to many of the changes between 2D and 3D culture is a real or perceived change in the concentration of elements (cytokines, tethered bioactive signaling, etc.) by the cell in its extracellular milieu. The complexity of how the concentration changes interact with cells to change cellular survival, attachment, and differentiation throughout central nervous system development is not yet well understood. Unraveling the effects of these concentration changes will ultimately lead to a better understanding of tissue development allowing us to begin to effectively manipulate the extracellular environment with scaffolds, drugs, stem cells, etc. to restore neurological function after traumatic brain and spinal cord injuries.