中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 61-63.doi: 10.4103/1673-5374.175043

• 观点:神经损伤修复保护与再生 • 上一篇    下一篇

超顺磁性氧化铁纳米粒子可促进神经再生

  

  • 收稿日期:2015-12-02 出版日期:2016-01-15 发布日期:2016-01-15
  • 基金资助:

    德国科学研究基金会资助 

Promoting neuronal regeneration using extracellular vesicles loaded with superparamagnetic iron oxide nanoparticles

Jenni Neubert, Jana Glumm   

  1. Institute of Cell Biology and Neurobiology, Center for Anatomy, Charité-Universitätsmedizin Berlin, Berlin, Germany(Neubert J, Glumm J)
    Department of Neurosurgery, HELIOS Klinikum Berlin Buch, Berlin, Germany (Glumm J)
  • Received:2015-12-02 Online:2016-01-15 Published:2016-01-15
  • Contact: Jana Glumm, M.D., jana.glumm@charite.de.
  • Supported by:

    This study was supported by DFG Grant KFO 213 to JG.

摘要:

德国柏林大学夏里特医学院解剖学中心细胞生物学及神经生物学系Jenni Neubert博士的研究结果表明,应用4种不同类型的超顺磁纳米颗粒对海马神经元形态产生的作用不同,取决于它们的表面涂层和各自的电荷,大小,浓度,以及细胞培养和培养条件。有趣的是,应用超顺磁纳米颗粒促进神经元-神经胶质混合培养的神经元神经突增生作用呈现出浓度和时间-效应关系。然而,这项假设还需要临床研究的证实。细胞外囊泡物质和它们运输可能会为促进大脑神经再生开辟新的思路。

Abstract:

Intercellular communication between neurons and glial cells via extracellular vesicles (EVs) as a novel mechanism of information transfer has been shown to be involved in regeneration processes within the central nervous system (CNS). Hence, to take advantage of EV signaling for therapeutic applications appears to be a completely new approach to promote regeneration. One fundamental reason why influencing neuronal recovery after damage remains problematic is due to limited access to molecular processes, which are essential for the transcription of growth-promoting cues. EVs are currently under investigation for their use as hybrid vectors containing both therapeutic agents and superparamagnetic iron oxide nanoparticles (SPIOs) that can be remote guided using an external magnetic field. SPIO binding and accumulation at the extracellular membrane and the subsequent internalization in EVs have to be ensured. It is also certainly necessary to characterize SPIO behavior under standardized conditions in cell cultures of individual cells as well as mixed cell cultures, for instance, containing neurons, astrocytes and microglial cells. Under these conditions, the possibility of influencing EV cargos and their delivery could indeed open up new strategies for affecting information transfer within the brain to promote neuroregeneration.