中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1701-1703.doi: 10.4103/1673-5374.194704

• 综述:神经损伤修复保护与再生 •    下一篇

神经功能恢复过程中神经元可塑性和大脑的连接

  

  • 出版日期:2016-11-30 发布日期:2016-11-30
  • 基金资助:
    这项工作得到德国研究委员会(HE3173 / 3-1)和德国学术交流服务和巴西国家技术与科学发展协会(TS Carvalho)的支持。

Methods for the analysis of neuronal plasticity and brain connectivity during neurological recovery

Eduardo H. Sanchez-Mendoza*, Tayana Silva de Carvalho, Dirk M. Hermann   

  1. Department of Neurology, University of Duisburg-Essen, Essen, Germany
  • Online:2016-11-30 Published:2016-11-30
  • Contact: Eduardo H. Sanchez-Mendoza, Ph.D., Eduardo.sanchez-mendoza@uk-essen.de.
  • Supported by:
    This work was supported by the German Research Council (HE3173/3-1) and the German Academic Exchange Service and the National Counsel of Technological and Scientifc Development from Brazil (to TS Carvalho).

摘要:

现在可以通过化学或生物衍生剂的使用建立神经系统疾病模型或检测可能的治疗方式。例如,可以通过化合物四氢吡啶外周给药诱导产生帕金森病,这种方法会诱导特定的多巴胺能神经元退变,并投射到纹状体黑质中。另一方面,许多潜在的治疗药物可通过多种方法外部给药以实现病变后的神经功能恢复。然而,血脑屏障通常会阻止这样的实验性药物到达大脑。而外周给药的另一不便之处在于,其可能会在实验动物进行诱导测试时产生不良行为影响。而研究神经元可塑性和机制以促进它在与临床相关的实验模型研究,显然已开始成为研究焦点。事实上,大量失败的实验已经研究了脑卒中后的神经保护策略,促进神经元可塑性在临床上或许是可以实现的,进而有效地帮助患者恢复。

orcid: 0000-0001-7461-8620 (Eduardo H. Sanchez-Mendoza)

Abstract: The study of neuronal plasticity under pathological conditions is now a major point of focus on the feld of neurological recovery. Afer the repeated failure of acute neuroprotection strategies for stroke treatment, the design of studies aimed at promoting the reconstruction of neuronal networks has become essential. Methods for the delivery of therapeutic agents on a steady dosage, thus preventing pharmacological peaks or excessive manipulation of experimental animals, are thus required. Additionally, methods that allow the visualization of neurological remodeling processes are fundamental to the understanding of how a therapeutic agent exerts its function. Here we describe how the use of miniosmotic pumps for the steady delivery of such agents, together with tract tracer injections, can be combined to unveil important information on how the brain changes afer stroke and how therapeutic agents promote brain remodeling recovery.

Key words: stroke, blood brain barrier, neuroplasticity, drug delivery