中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1804-1809.doi: 10.4103/1673-5374.194750

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

膜结合蛋白Ankfy1、在神经发育过程中重要吗?

  

  • 出版日期:2016-11-30 发布日期:2016-11-30
  • 基金资助:

    湖北省卫计委基金(WJ2015MA007)

Ankfy1 is dispensable for neural stem/precursor cell development

Chao Weng1, Man Ding1, Lian-sheng Chang2, Ming-xin Ren2, Hong-feng Zhang3, Zu-neng Lu1, *, Hui Fu2, 4, *   

  1. 1 Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China 2 Department of Anatomy and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, China 3 Department of Pathology, Central Hospital of Wuhan, Wuhan, Hubei Province, China 4 Hubei-MOST KLOS & KLOBME, Wuhan, Hubei Province, China
  • Online:2016-11-30 Published:2016-11-30
  • Contact: Hui Fu, Ph.D. or Zu-neng Lu, M.D., hueyfu@yahoo.com or lzn196480@126.com.
  • Supported by:

    Dr. Hui Fu was supported by the National Natural Science Foundation of China, No.81371338, and by Open Research Fund Program of Hubei-MOST KLOS & KLOBME. Dr. Zu-neng Lu was supported by grants from Health and Family Planning Commission of Hubei Province scientifc research project, No. WJ2015MA007.

摘要:

Ankfy1基因编码的是一种膜结合蛋白,既往研究较少。我们发现它在早期发育过程中在神经干/祖细胞中特异性地表达。为了进一步探讨其在神经发育过程中的功能,运用遗传背景为Balb/C和C57/BL6的小鼠,我们培育了Ankfy1基因敲除小鼠。在混合遗传背景中,免疫荧光、原位杂交等检测显示,Ankfy1基因敲除小鼠在发育过程中没有表现出神经功能缺损症状,2月龄成年小鼠也未发现神经功能缺损症状。然而,在经过7次逆代交配的C57/BL6近交系小鼠的遗传背景,Ankfy1基因敲除小鼠在胚胎时期便死亡,在E11.5及胚胎更早时期,我们运用PCR基因鉴定,未能得到任何纯合子Ankfy1基因敲除小鼠胚胎。结果可以说明,Ankfy1蛋白在神经干/祖细胞的发育过程中并不是必需的,但可能是早期胚胎发育过程中的关键蛋白,这取决于不同的遗传背景。 

orcid: 0000-0003-0252-6009 (Hui Fu), 0000-0003-0001-3437 (Zu-neng Lu)

关键词: 神经再生, Ankfy1, 神经发育, 遗传背景, 蛋白, 功能, 基因敲除, 神经干细胞/前体细胞, 胚胎, 发育

Abstract:

There are few studies on the membrane protein Ankfy1. We have found Ankfy1 is specifcally expressed in neural stem/precursor cells during early development in mice (murine). To further explore Ankfy1 function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immuno?uorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfy1 null mice during development and in adults up to 2 months old. However, Ankfy1 gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even afer seven generations of backcrossing. Polymerase chain reaction confrmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfy1 protein is dispensable in neural stem/precursor cells, but could be critical for early embryonic murine development, depending on the genetic background.

Key words: nerve regeneration, Ankfy1, neural development, genetic background, protein, function, gene knockout, neural stem/precursor cells, embryo, neural regeneration