中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (7): 1036-1039.doi: 10.4103/1673-5374.211175

• 综述:神经损伤修复保护与再生 • 上一篇    下一篇

活化C激酶受体1调节神经发育

  

  • 收稿日期:2017-06-26 出版日期:2017-07-15 发布日期:2017-07-15
  • 基金资助:

    本次研究得到了美国国立卫生研究院(NINDS;基金号R15NS098389)的支持。

RACK1 regulates neural development

Leah Kershner1, Kristy Welshhans1, 2   

  1. 1 Department of Biological Sciences, Kent State University, Kent, OH, USA; 2 School of Biomedical Sciences, Kent State University, Kent, OH, USA
  • Received:2017-06-26 Online:2017-07-15 Published:2017-07-15
  • Contact: Kristy Welshhans, Ph.D., kwelshha@kent.edu.
  • Supported by:

    Research in the author's laboratory that is related to this review article is supported by a grant from NIH (NINDS; grant number R15NS098389 to KW).

摘要:

 

活化C激酶受体1(RACK1)对于神经发育至关重要,缺乏RACK1可导致胚胎期小鼠是死亡;然而,关于其在神经系统发育中的作用尚未得到完全阐释。
本研究表明,RACK1直接调控原代小鼠皮质神经元的轴突生长。首先,研究使用shRNA敲除RACK1,导致轴突变短,抵消了BDNF诱导的生长锥面积增加,这表明RACK1在调节生长锥形态中起着重要作用。有趣的是,RACK1过表达也导致轴突变短,这表明适当水平的RACK1表达对于轴突生长是必需的。RACK1的过度表达也导致生长锥形态异常。

总之,文中发现RACK1在正常和BDNF刺激条件下对于适当的轴突长度和形态是至关重要的。总之,这些研究显示了RACK1在调节神经发育中的明确作用。

ORCID:0000-0001-6624-4719(Kristy Welshhans)

Abstract:

Receptor for activated C kinase 1 (RACK1) is an evolutionarily conserved scaffolding protein within the tryptophan-aspartate (WD) repeat family of proteins. RACK1 can bind multiple signaling molecules concurrently, as well as stabilize and anchor proteins. RACK1 also plays an important role at focal adhesions, where it acts to regulate cell migration. In addition, RACK1 is a ribosomal binding protein and thus, regulates translation. Despite these numerous functions, little is known about how RACK1 regulates nervous system development. Here, we review three studies that examine the role of RACK1 in neural development. In brief, these papers demonstrate that (1) RACK-1, the C. elegans homolog of mammalian RACK1, is required for axon guidance; (2) RACK1 is required for neurite extension of neuronally differentiated rat PC12 cells; and (3) RACK1 is required for axon outgrowth of primary mouse cortical neurons. Thus, it is evident that RACK1 is critical for appropriate neural development in a wide range of species, and future discoveries could reveal whether RACK1 and its signaling partners are potential targets for treatment of neurodevelopmental disorders or a therapeutic approach for axonal regeneration.

Key words: RACK1, RACK-1, neural development, neurite outgrowth, axon outgrowth, axon guidance