中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (8): 1510-1517.doi: 10.4103/1673-5374.257532

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

针刺促进脑出血恢复:与上调神经营养因子表达有关?

  

  • 出版日期:2020-08-15 发布日期:2020-09-16

Acupuncture promotes functional recovery after cerebral hemorrhage by upregulating neurotrophic factor expression

Dan Li2 , Qiu-Xin Chen1 , Wei Zou1, * , Xiao-Wei Sun1 , Xue-Ping Yu1 , Xiao-Hong Dai1 , Wei Teng1   

  1. 1 Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China 2 Department of Acupuncture, Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China
  • Online:2020-08-15 Published:2020-09-16
  • Contact: Wei Zou, MD, kuangzuo2013@sina.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81473764, 81273824, 30772840 (to WZ); the Doctoral Fund of Ministry of Education of China, No. 20102327110003 (to WZ); the Natural Science Foundation of Heilongjiang Province of China, No. ZD201204 (to WZ); the Special Fund for Technological Innovation Research of Harbin of China, No. 2012RFXXS062 (to WZ); the Doctoral Innovation Fund of Heilongjiang University of Chinese Medicine of China, No. 2015bs03 (to QXC); the Chunhui Plans Research Cooperation Project of China, No. Z2007-1-15010 (to WZ), the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province of China, No. UNPYSCT-2018234 (to QXC).

摘要: orcid: 0000-0001-9391-7181 (Wei Zou)

Abstract: Acupuncture is widely used in the treatment of cerebral hemorrhage, and it improves outcomes in experimental animal models and patients. However, the mechanisms underlying the effectiveness of acupuncture treatment for cerebral hemorrhage are still unclear. In this study, a model of intracerebral hemorrhage was produced by injecting 50 μL autologous blood into the caudate nucleus in Wistar rats. Acupuncture at Baihui (DU20) and Qubin (GB7) acupoints was performed at a depth of 1.0 inch, 12 hours after blood injection, once every 24 hours. The needle was rotated at 200 r/min for 5 minutes, For each 30-minute session, needling at 200 r/min was performed for three sessions, each lasting 5 minutes. For the positive control group, at 6 hours, and 1, 2, 3 and 7 days after induction of hemorrhage, the rats were intraperitoneally injected with 1 mL aniracetam (0.75 mg/mL), three times a day. The Bederson behavioral test was used to assess palsy in the contralateral limbs. Western blot assay was used to examine the expression levels of Nestin and basic fibroblast growth factor in the basal ganglia. Immunohistochemistry was performed to count the number of Nestin- and glial cell line-derived neurotrophic factor-positive cells in the basal ganglia. Acupuncture effectively reduced hemorrhage and brain edema, elevated the expression levels of Nestin and basic fibroblast growth factor in the basal ganglia, and increased the number of Nestin- and glial cell line-derived neurotrophic factor-positive cells in the basal ganglia. Together, these findings suggest that acupuncture promotes functional recovery after cerebral hemorrhage by increasing the expression of neurotrophic factors. The study was approved by the Committee for Experimental Animals of Heilongjiang Medical Laboratory Animal Center (approval No. 2017061001) on June 10, 2017.

Key words: acupuncture, basic fibroblast growth factor, brain cell protection, cerebral hemorrhage, electron microscope, glial cell line-derived neurotrophic factor, immunohistochemistry, Nestin, western blot assay