中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (4): 573-585.doi: 10.4103/1673-5374.266907

• 综述:神经损伤修复保护与再生 •    下一篇

用于神经元分化的细胞外基质和仿生工程微环境

  

  • 出版日期:2020-04-15 发布日期:2020-05-28

Extracellular matrix and biomimetic engineering microenvironment for neuronal differentiation

Deepak Jain1, Sabrina Mattiassi1, Eyleen L. Goh2, Evelyn K.F. Yim1   

  1. 1 Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada
    2 Neuroscience Academic Clinical Programme, Duke-NUS Medical School, Singapore
  • Online:2020-04-15 Published:2020-05-28
  • Contact: Evelyn K.F. Yim, PhD,eyim@uwaterloo.ca.
  • Supported by:
    The work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery (NSERC 2016040; to DJ, SM and EKFY) and University of Waterloo start up fund (to DJ, SM and EKFY) for their generous funding. In addition, NSERC Undergraduate Student Research Awards (USRA; to SM and EKFY) and Collaborative Research and Training Experience (CREATE, 401207296; to SM and EKFY) for their generous partial funding.

摘要:

orcid: 0000-0001-9812-1801 (Evelyn K.F. Yim) 

         0000-0002-0199-2473 (Deepak Jain) 

         0000-0002-7312-6427 (Sabrina Mattiassi) 

         0000-0002-8244-6959 (Eyleen L. Goh)

Abstract: Extracellular matrix (ECM) influences cell differentiation through its structural and biochemical properties. In nervous system, neuronal behavior is influenced by these ECMs structures which are present in a meshwork, fibrous, or tubular forms encompassing specific molecular compositions. In addition to contact guidance, ECM composition and structures also exert its effect on neuronal differentiation. This short report reviewed the native ECM structure and composition in central nervous system and peripheral nervous system, and their impact on neural regeneration and neuronal differentiation. Using topographies, stem cells have been differentiated to neurons. Further, focussing on engineered biomimicking topographies, we highlighted the role of anisotropic topographies in stem cell differentiation to neurons and its recent temporal application for efficient neuronal differentiation.

Key words: biomimetic platforms, biophysical cues, contact guidance, extracellular matrix, neuronal development, neural regeneration, neural stem cell niche, neuronal differentiation, neuronal maturation, stem cell, topography