中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (8): 1560-1567.doi: 10.4103/1673-5374.274348

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

全横断脊髓损伤中长链非编码RNA的表达谱：微阵列分析

  

  • 出版日期:2020-08-15 发布日期:2020-09-16

Expression of long non-coding RNAs in complete transection spinal cord injury: a transcriptomic analysis

Lu Ding1, 2, #, Wen-Jin Fu3, #, Hong-Yan Di4, #, Xiao-Min Zhang1 , Yu-Tian Lei5 , Kang-Zhen Chen1 , Tao Wang6 , Hong-Fu Wu1, *   

  1. 1 Institute of Stem Cells and Regenerative Medicine, Department of Physiology, Guangdong Medical University, Dongguan, Guangdong Province, China 2 Scientific Research Center, the Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, China 3 Clinical Laboratory, Houjie Hospital of Guangdong Medical University, Dongguan, Guangdong Province, China 4 Department of Neurology, Dalingshan Hospital, Dongguan, Guangdong Province, China 5 Department of Hand & Foot Surgery, Houjie Hospital of Guangdong Medical University, Dongguan, Guangdong Province, China 6 Department of Surgery, the Third Hospital of Guangdong Medical University (Longjiang Hospital of Shunde District), Foshan, Guangdong Province, China
  • Online:2020-08-15 Published:2020-09-16
  • Contact: Hong-Fu Wu, PhD, hongfuw@126.com.
  • Supported by:
    This work was financially supported by the National Natural Science Foundation of China, No. 81371366 (to HFW); Characteristic Innovation Project of Colleges and Universities in Guangdong Province of China, No. 2018KTSCX075 (to HFW); the Key Project of Social Development of Dongguan of China, No. 20185071521640 (to HFW); College Students’ Science and Technology Innovation Training Project, China, Nos. 201810571058, GDMU2018024, GDMU2018056, GDMU2018061 (to HFW); College Students’ Innovative Experimental Project in Guangdong Medical University, China, No. ZZDS001 (to HFW); College Students’ Science and Technology Innovation Cultivation Project in Guangdong of China, No. pdjh2019b0217 (to HFW).

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摘要： orcid: 0000-0002-1115-3681 (Hong-Fu Wu)

Abstract: Long non-coding RNAs (lncRNAs) are abundantly expressed in the central nervous system and exert a critical role in gene regulation via multiple biological processes. To uncover the functional significance and molecular mechanisms of lncRNAs in spinal cord injury (SCI), the expression signatures of lncRNAs were profiled using RNA sequencing (RNA-seq) technology in a Sprague-Dawley rat model of the 10th thoracic vertebra complete transection SCI. Results showed that 116 of 14,802 detected lncRNAs were differentially expressed, among which 16—including eight up-regulated (H19, Vof16, Hmox2-ps1, LOC100910973, Ybx1-ps3, Nnat, Gcgr, LOC680254) and eight down-regulated (Rmrp, Terc, Ngrn, Ppp2r2b, Cox6a2, Rpl37a-ps1, LOC360231, Rpph1)—demonstrated fold changes > 2 in response to transection SCI. A subset of these RNA-seq results was validated by quantitative real-time PCR. The levels of 821 mRNAs were also significantly altered post-SCI; 592 mRNAs were up-regulated and 229 mRNAs were down-regulated by more than 2-fold. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that differentially expressed mRNAs were related to GO biological processes and molecular functions such as injury and inflammation response, wound repair, and apoptosis, and were significantly enriched in 15 KEGG pathways, including cell phagocytosis, tumor necrosis factor alpha pathway, and leukocyte migration. Our results reveal the expression profiles of lncRNAs and mRNAs in the rat spinal cord of a complete transection model, and these differentially expressed lncRNAs and mRNAs represent potential novel targets for SCI treatment. We suggest that lncRNAs may play an important role in the early immuno-inflammatory response after spinal cord injury. This study was approved by the Administration Committee of Experimental Animals, Guangdong Province, China.

Key words: cell apotosis, complete transection injury, high throughput sequencing, inflammation, ischemia related factor vof-16, long non-coding RNA, secondary damage, spinal cord, TNF signaling, transcriptomes