中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (8): 1526-1531.doi: 10.4103/1673-5374.274349

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

枸杞多糖可保护视网膜神经节细胞免受氧化应激损伤

  

  • 出版日期:2020-08-15 发布日期:2020-09-16

Lycium barbarum polysaccharides protects retinal ganglion cells against oxidative stress injury

Lian Liu1 , Xiao-Yuan Sha1 , Yi-Ning Wu2 , Meng-Ting Chen1 , Jing-Xiang Zhong1, *   

  1. 1 Department of Ophthalmology, Affiliated First Hospital of Jinan University, Guangzhou, Guangdong Province, China 2 Department of Ophthalmology, Guangdong Women and Children Hospital, Guangzhou, Guangdong Province, China
  • Online:2020-08-15 Published:2020-09-16
  • Contact: Jing-Xiang Zhong, PhD, zjx85221206@126.com.
  • Supported by:
    This work was supported by grants from Project of Administration of Traditional Chinese Medicine of Guangdong Province of China, No. 20161071 (to LL), and Medical Scientific Research Foundation of Guangdong Province of China, No. A2019098 (to LL).

摘要: orcid: 0000-0002-5774-7602 (Jing-Xiang Zhong)

Abstract: The accumulation of excessive reactive oxygen species can exacerbate any injury of retinal tissue because free radicals can trigger lipid peroxidation, protein damage and DNA fragmentation. Increased oxidative stress is associated with the common pathological process of many eye diseases, such as glaucoma, diabetic retinopathy and ischemic optic neuropathy. Many studies have demonstrated that Lycium barbarum polysaccharides (LBP) protects against oxidative injury in numerous cells and tissues. For the model of hypoxia we used cultured retinal ganglion cells and induced hypoxia by incubating with 200 µM cobalt chloride (CoCl2) for 24 hours. To investigate the protective effect of LBP and its mechanism of action against oxidative stress injury, the retinal tissue was pretreated with 0.5 mg/mL LBP for 24 hours. The results of flow cytometric analysis showed LBP could effectively reduce the CoCl2-induced retinal ganglion cell apoptosis, inhibited the generation of reactive oxygen species and the reduction of mitochondrial membrane potential. These findings suggested that LBP could protect retinal ganglion cells from CoCl2-induced apoptosis by reducing mitochondrial membrane potential and reactive oxygen species.

Key words: caspase, cell apoptosis, cobalt chloride, Lycium barbarum polysaccharides, mitochondrial membrane potential, oxidative stress injury, reactive oxygen species, retinal ganglion cells