中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (10): 1962-1968.doi: 10.4103/1673-5374.280328

• 原著:视神经损伤修复保护与再生 • 上一篇    

早产儿视网膜病变中长链非编码RNA的表达谱

  

  • 出版日期:2020-10-15 发布日期:2020-08-19
  • 基金资助:
    国家自然科学基金项目(81600747),辽宁省医生创业基金(201501020

Expression profiles of long noncoding RNAs in retinopathy of prematurity

Yue Wang, Xue Wang, Yuan Ma, Yue-Xia Wang, Yu Di   

  1. Department of Ophthalmology, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, China
  • Online:2020-10-15 Published:2020-08-19
  • Contact: Yu Di, MD, diyu81@126.com.
  • Supported by:
    The work was supported by the National Natural Science Foundation of China, No. 81600747 (to YD) and Startup Foundation for Doctors of Liaoning Province, China, No. 201501020 (to YD).

摘要:

长链非编码RNA可调节糖尿病性视网膜病变中视网膜内皮细胞的增殖、迁移与视网膜新生血管形成,由于早产儿视网膜病变与糖尿病性视网膜神经变性的发病机制相似,因而长链非编码RNA也可能在早产儿视网膜病变中起作用。实验对7d龄小鼠高氧(75±2%)饲养5d,常氧饲养5d建立早产儿视网膜病变模型,采用高通量测序技术检测小鼠视网膜组织中lncRNA和mRNA的表达,然后通过GO和KEGG分析其生物学功能。发现:(1)与正常小鼠相比,模型小鼠存在57种差异表达的lncRNA,其中43种上调,14种下调;42种mRNA差异表达,其中24种上调,18种下调。差异表达的基因主要涉及眼部发育和代谢;(2)差异表达的lncRNA可能通过微小RNA和多种信号通路调节视网膜神经变,提示这个差异表达的长链非编码RNA可能成为早产儿视网膜病变的治疗靶点。实验于2016-2-25经盛京医院伦理委员会批准(伦理号2016PS074K)。

orcid: 0000-0001-6664-2952 (Yu Di)

关键词: 早产儿视网膜病变, 神经血管疾病, 视网膜发育, 小胶质细胞, 信号通路, 视神经病变, 生物信息学, 基因治疗, 视网膜新血管形成, 长链非编码RNA

Abstract: Long noncoding RNA (lncRNA) regulates the proliferation and migration of human retinal endothelial cells, as well as retinal neovascularization in diabetic retinopathy. Based on similarities between the pathogenesis of retinopathy of prematurity (ROP) and diabetic retinopathy, lncRNA may also play a role in ROP. Seven-day-old mice were administered 75 ± 2% oxygen for 5 days and normoxic air for another 5 days to establish a ROP model. Expression of lncRNA and mRNA in the retinal tissue of mice was detected by high-throughput sequencing technology, and biological functions of the resulted differentially expressed RNAs were evaluated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. The results showed that compared with the control group, 57 lncRNAs were differentially expressed, including 43 upregulated and 14 downregulated, in the retinal tissue of ROP mice. Compared with control mice, 42 mRNAs were differentially expressed in the retinal tissue of ROP mice, including 24 upregulated and 18 downregulated mRNAs. Differentially expressed genes were involved in ocular development and related metabolic pathways. The differentially expressed lncRNAs may regulate ROP in mice via microRNAs and multiple signaling pathways. Our results revealed that these differentially expressed lncRNAs may be therapeutic targets for ROP treatment. This study was approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University on February 25, 2016 (approval No. 2016PS074K).

Key words: bioinformatics, gene therapy, long noncoding RNA, microglial, neurovascular disease, optic neuropathy, retinal development, retinal neovascularization, retinopathy of prematurity, signaling pathways