中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (10): 2311-2315.doi: 10.4103/1673-5374.336873

• 原著:神经再生技术 • 上一篇    下一篇

超小超顺磁氧化铁纳米颗粒标记鼠原代培养单核细胞

  

  • 出版日期:2022-10-15 发布日期:2022-03-16
  • 基金资助:
    德国研究基金会(DFG)赠款Klinische Forschergruppe(KFO)213的支持

Magnetic labeling of primary murine monocytes using very small superparamagnetic iron oxide nanoparticles

Martin Pohland1, Christoph Pohland1, Jürgen Kiwit2, Jana Glumm1, 2   

  1. 1Institute of Cell Biology and Neurobiology, Center for Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany; 2Department of Neurosurgery, Helios Klinikum Berlin Buch, Berlin, Germany
  • Online:2022-10-15 Published:2022-03-16
  • Contact: Jana Glumm, MD, jana.glumm@charite.de.
  • Supported by:
    This work was supported by Deutsche Forschungsgemeinschaft (DFG) grant Klinische Forschergruppe (KFO) 213 (to JG).

摘要:

纳米颗粒由于尺寸非常小,可以与中枢神经系统的所有细胞产生相互作用,其中最有应用潜力的纳米粒子是超小超顺磁氧化铁纳米颗粒(VSOP),它有柠檬酸盐涂层以实现静电稳定。实验旨在探讨柠檬酸盐包裹的VSOP(直径6.5–7.5 nm和7.5–8.7 nm)和羧基葡聚糖包裹的超顺磁性氧化铁纳米颗粒(SPIO)Resovist对容易进入受伤的中枢神经系统的小鼠血源性单核细胞的影响。通过评估小鼠血源性单核细胞的活力、细胞因子和趋化因子的分泌以及铁的吸收的影响。实验发现(1)单核细胞积聚了VSOP和Resovist,(2)这种吸收是纳米颗粒和时间依赖性的,(3)单核细胞活力的下降与治疗有关,(4)VSOP和Resovist的孵化不改变细胞因子的平衡,(5)总体而言,用0.75mM的直径6.5–7.5 nm的VSOP处理6h可能足以有效地标记单核细胞。用VSOP标记血源性单核细胞是安全的。VSOP标记的单核细胞可用于进一步研究受伤的中枢神经系统,例如用携带药物的VSOP。

https://orcid.org/0000-0001-5756-3718 (Jana Glumm) 

Abstract: Due to their very small size, nanoparticles can interact with all cells in the central nervous system. One of the most promising nanoparticle subgroups are very small superparamagnetic iron oxide nanoparticles (VSOP) that are citrate coated for electrostatic stabilization. To determine their influence on murine blood-derived monocytes, which easily enter the injured central nervous system, we applied VSOP and carboxydextran-coated superparamagnetic iron oxide nanoparticles (Resovist). We assessed their impact on the viability, cytokine, and chemokine secretion, as well as iron uptake of murine blood-derived monocytes. We found that (1) the monocytes accumulated VSOP and Resovist, (2) this uptake seemed to be nanoparticle- and time-dependent, (3) the decrease of monocytes viability was treatment-related, (4) VSOP and Resovist incubation did not alter cytokine homeostasis, and (5) overall a 6-hour treatment with 0.75 mM VSOP-R1 was probably sufficient to effectively label monocytes for future experiments. Since homeostasis is not altered, it is safe to label blood-derived monocles with VSOP. VSOP labeled monocytes can be used to study injured central nervous system sites further, for example with drug-carrying VSOP.

Key words: CD11b, cytokine, Ferucarbotran, Mac1, MPS, MRI, Resovist, superparamagnetic iron oxide nanoparticles (SPIO), very small superparamagnetic iron oxide nanoparticles (VSOP), viability