中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (2): 289-292.doi: 10.4103/1673-5374.346465

• 综述:脑损伤修复保护与再生 • 上一篇    下一篇

体外模拟创伤性脑损伤:开发高通量模型以测试基于生物材料的疗法

  

  • 出版日期:2023-02-15 发布日期:2022-08-05

Simulating traumatic brain injury in vitro: developing high throughput models to test biomaterial based therapies

Raja Haseeb Basit1, 2, Jessica Wiseman2, 4, Farhana Chowdhury3, 4, Divya Maitreyi Chari4, *   

  1. 1Bradford Royal infirmary, Bradford, West Yorkshire, UK; 2Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK; 3Labcorp Drug Development, Harrogate, UK; 4Neural Tissue Engineering Keele Group, School of Medicine, Keele University, Staffordshire, UK
  • Online:2023-02-15 Published:2022-08-05
  • Contact: Divya Maitreyi Chari, MD, d.chari@keele.ac.uk.
  • Supported by:
    Work contributing to the development of the models described in this article was funded by awards from the EPSRC Doctoral Training Centre in Regenerative Medicine and an NHS bursary.

摘要: https://orcid.org/0000-0002-7095-8578 (Divya Maitreyi Chari)

Abstract: Traumatic brain injuries are serious clinical incidents associated with some of the poorest outcomes in neurological practice. Coupled with the limited regenerative capacity of the brain, this has significant implications for patients, carers, and healthcare systems, and the requirement for life-long care in some cases. Clinical treatment currently focuses on limiting the initial neural damage with long-term care/support from multidisciplinary teams. Therapies targeting neuroprotection and neural regeneration are not currently available but are the focus of intensive research. Biomaterial-based interventions are gaining popularity for a range of applications including biomolecule and drug delivery, and to function as cellular scaffolds. Experimental investigations into the development of such novel therapeutics for traumatic brain injury will be critically underpinned by the availability of appropriate high throughput, facile, ethically viable, and pathomimetic biological model systems. This represents a significant challenge for researchers given the pathological complexity of traumatic brain injury. Specifically, there is a concerted post-injury response mounted by multiple neural cell types which includes microglial activation and astroglial scarring with the expression of a range of growth inhibitory molecules and cytokines in the lesion environment. Here, we review common models used for the study of traumatic brain injury (ranging from live animal models to in vitro systems), focusing on penetrating traumatic brain injury models. We discuss their relative advantages and drawbacks for the developmental testing of biomaterial-based therapies.

Key words: astroglial scar, biomaterial, cortical culture, in vitro model, microglial infiltration, multicellular model, penetrating injury, scaffold, traumatic brain injury