中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (2): 428-433.doi: 10.4103/1673-5374.346469

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

C2脊髓半切对大鼠呼吸和心血管功能的影响

  

  • 出版日期:2023-02-15 发布日期:2022-08-09

Effects of C2 hemisection on respiratory and cardiovascular functions in rats

Pauline Michel-Flutot1, Arnaud Mansart2, Abdallah Fayssoil1,  3, Stéphane Vinit1, *   

  1. 1Université Paris-Saclay, UVSQ, Inserm, END-ICAP, Versailles, France; 2Université Paris-Saclay, UVSQ, Inserm, Infection et Inflammation (2I), Versailles, France; 3Raymond Poincaré Hospital, AP-HP, Garches, France
  • Online:2023-02-15 Published:2022-08-09
  • Contact: Stéphane Vinit, PhD, HDR, stephane.vinit@uvsq.fr.
  • Supported by:
    This work was supported by funding from the Chancellerie des Universités de Paris (Legs Poix) (to SV), Fondation Medisite (to SV), INSERM (to SV, AM, AF) and Université de Versailles Saint-Quentin-en-Yvelines (to SV, AM, AF).

摘要:

高位脊髓损伤可诱发永久性的神经运动和自主神经障碍,这些损伤通过调控交感神经系统影响中枢呼吸和心血管功能。为评估C2脊髓半切对大鼠伤时和伤后7d代表生命机能的不同参数(即呼吸功能、心血管和肾脏滤过参数)的影响,实验在大鼠伤后7d的安静呼吸过程中,用胸腔镜评估的通气参数没有受到影响,而通过超声波观察和膈肌肌电图确认了永久性横膈膜轻瘫。有趣的是,C2半切后平均动脉压立即降低,并在伤后7d完全恢复。伤后7d,肾脏滤过功能未受影响;然而,以左心室射血分数降低为特征的残余收缩功能持续存在。这些结果表明,在C2半切后,膈肌活动和收缩功能在伤后7d内受到影响,而呼吸和心血管系统显示出巨大的适应性,以维持通气参数和血压平衡,后者可能由最初受伤时幸免于难的剩余降交感神经输入维持。对高位脊髓损伤的生理病理进行更好的广泛描述可能有利于评估提高心肺功能恢复治疗方法的效果。

https://orcid.org/0000-0001-7013-1741 (Stéphane Vinit)

Abstract: High cervical spinal cord injuries induce permanent neuromotor and autonomic deficits. These injuries impact both central respiratory and cardiovascular functions through modulation of the sympathetic nervous system. So far, cardiovascular studies have focused on models of complete contusion or transection at the lower cervical and thoracic levels and diaphragm activity evaluations using invasive methods. The present study aimed to evaluate the impact of C2 hemisection on different parameters representing vital functions (i.e., respiratory function, cardiovascular, and renal filtration parameters) at the moment of injury and 7 days post-injury in rats. No ventilatory parameters evaluated by plethysmography were impacted during quiet breathing after 7 days post-injury, whereas permanent diaphragm hemiplegia was observed by ultrasound and confirmed by diaphragmatic electromyography in anesthetized rats. Interestingly, the mean arterial pressure was reduced immediately after C2 hemisection, with complete compensation at 7 days post-injury. Renal filtration was unaffected at 7 days post-injury; however, remnant systolic dysfunction characterized by a reduced left ventricular ejection fraction persisted at 7 days post-injury. Taken together, these results demonstrated that following C2 hemisection, diaphragm activity and systolic function are impacted up to 7 days post-injury, whereas the respiratory and cardiovascular systems display vast adaptation to maintain ventilatory parameters and blood pressure homeostasis, with the latter likely sustained by the remaining descending sympathetic inputs spared by the initial injury. A better broad characterization of the physiopathology of high cervical spinal cord injuries covering a longer time period post-injury could be beneficial for understanding evaluations of putative therapeutics to further increase cardiorespiratory recovery. 

Key words: C2 spinal cord injury, cardiovascular, diaphragm activity, heart function, hemiplegia, rat model, respiratory, ultrasound