中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (4): 895-900.doi: 10.4103/1673-5374.353505

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

振荡电场刺激促进神经干细胞神经生成修复大鼠脊髓损伤

  

  • 出版日期:2023-04-15 发布日期:2022-10-29

Oscillating field stimulation promotes neurogenesis of neural stem cells through miR-124/Tal1 axis to repair spinal cord injury in rats

Chao Fang1, Jian Sun2, Jun Qian1, *, Cai-Liang Shen1, *   

  1. 1Department of Orthopedics & Spine Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; 2Department of Orthopedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China
  • Online:2023-04-15 Published:2022-10-29
  • Contact: Cai-Liang Shen, MD, shencailiang@ahmu.edu.cn; Jun Qian, MD, qjpaper@sina.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81471273 (to JQ), and 81472088 (to CLS); the Natural Science Research Projects in Colleges and Universities of Anhui Province, No. KJ2020ZD23 (to JQ); the Natural Science Foundation of Anhui Province, No. 2208085MH210 (to JQ).

摘要:

该团队过去的研究已经证实,振荡电场能够促进大鼠脊髓内源性神经干细胞向神经元分化,从而提高大鼠脊髓损伤后运动功能的恢复。此次,为进一步探究了振荡电场与神经干细胞移植的协同作用及其机制,实验首先通过体外实验发现振荡电场能够通过调控microRNA-124/Tal1通路,促进体外培养的神经干细胞的神经发生。接下来的体内实验将神经干细胞移植入大鼠模型的脊髓损伤部位,并以振荡电场刺激进行干预,结果显示振荡电场刺激可有效促进移植神经干细胞在体内的神经发生,大鼠的运动功能也得到了进一步的提高。最后,振荡电场联合神经干细胞移植还能有效减少脊髓损伤部位空洞的形成。振荡电场刺激联合神经干细胞移植可通过miR-124/Tal1轴促进脊髓损伤后的功能恢复。

https://orcid.org/0000-0002-9835-6384 (Cai-Liang Shen)

Abstract: Spinal cord injury often leads to severe motor and sensory deficits, and prognosis using the currently available therapies remains poor. Therefore, we aimed to explore a novel therapeutic approach for improving the prognosis of spinal cord injury. In this study, we implanted oscillating field stimulation devices and transplanted neural stem cells into the thoracic region (T9–T10) of rats with a spinal cord contusion. Basso-Beattie-Bresnahan scoring revealed that oscillating field stimulation combined with neural stem cells transplantation promoted motor function recovery following spinal cord injury. In addition, we investigated the regulation of oscillating field stimulation on the miR-124/Tal1 axis in neural stem cells. Transfection of lentivirus was performed to investigate the role of Tal1 in neurogenesis of neural stem cells induced by oscillating field stimulation. Quantitative reverse transcription-polymerase chain reaction, immunofluorescence and western blotting showed that oscillating field stimulation promoted neurogenesis of neural stem cells in vitro and in vivo. Hematoxylin and eosin staining showed that oscillating field stimulation combined with neural stem cells transplantation alleviated cavities formation after spinal cord injury. Taking the results together, we concluded that oscillating field stimulation decreased miR-124 expression and increased Tal1 content, thereby promoting the neurogenesis of neural stem cells. The combination of oscillating field stimulation and neural stem cells transplantation improved neurogenesis, and thereby promoted structural and functional recovery after spinal cord injury.

Key words: miR-124, neural stem cell, neurogenesis, oscillating field stimulation, recovery, spinal cord injury, Tal1, tissue repair, transplantation