中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (6): 1273-1274.doi: 10.4103/1673-5374.360171

• 观点:神经损伤修复保护与再生 • 上一篇    下一篇

人神经前体细胞中 Wnt/β-catenin 信号通路的药理学操作改变了它们的分化潜能和神经元产量

  

  1. 弗拉姆眼科研究所,罗切斯特大学,美国
  • 出版日期:2023-06-15 发布日期:2022-12-22

Neural differentiation protocols: how to choose the correct approach

Michael Telias*   

  1. Flaum Eye Institute, University of Rochester, Rochester, NY, USA
  • Online:2023-06-15 Published:2022-12-22
  • Contact: Michael Telias, MSc, PhD, Michael_Telias@urmc.rochester.edu.
  • Supported by:
    The present work was supported by an unrestricted grant to the University of Rochester’s Department of Ophthalmology from the Research to Prevent Blindness Foundation. 

摘要: https://orcid.org/0000-0002-7632-6942 (Michael Telias) 

Abstract: Pluripotent stem cells in neural differentiation: characterization and potential: The establishment and use of pluripotent stem cells (PSCs), including embryonic (ESCs) and induced (iPSCs), constitutes a major scientific breakthrough of the last decades. Human PSCs hold the potential to deliver regenerative therapies in many diseases, including neurological ones. The general approach is to produce functioning human neurons and glial cells in vitro, to be later implanted in the diseased nervous system, replacing dysfunctional or dead cells. In addition, human and other animal-sourced PSCs make useful and dynamic in vitro models for neurodevelopmental, neurodegenerative and psychiatric disorders, enabling researchers to continuously produce normal and diseased neurons to investigate basic mechanistic questions, which can eventually lead to new therapeutics. Therefore, the development of efficient protocols to induce neural differentiation in ESCs and iPSCs is currently a major effort in the field (Mertens et al., 2016).