中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (2): 302-308.doi: 10.4103/1673-5374.379038

• 综述:神经损伤修复保护与再生 • 上一篇    下一篇

5-溴-2´-脱氧尿苷标记:历史观点、影响检测的因素、毒性及其在神经发生中的意义

  

  • 出版日期:2024-02-15 发布日期:2023-08-30

5-Bromo-2'-deoxyuridine labeling: historical perspectives, factors influencing the detection, toxicity, and its implications in the neurogenesis

Joaquín Martí-Clúa*   

  1. Unidad de Citología e Histología. Departament de Biologia Cel·lular, de Fisiologia i d’Immunologia. Facultad de Biociencias. Institut de Neurociències. Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
  • Online:2024-02-15 Published:2023-08-30
  • Contact: Joaquín Martí-Clúa, joaquim.marti.clua@uab.es.

摘要: https://orcid.org/0000-0002-6774-0092 (Joaquín Martí Clua)

Abstract: The halopyrimidine 5-bromo-2′-deoxyuridine (BrdU) is an exogenous marker of DNA synthesis. Since the introduction of monoclonal antibodies against BrdU, an increasing number of methodologies have been used for the immunodetection of this synthesized bromine-tagged base analogue into replicating DNA. BrdU labeling is widely used for identifying neuron precursors and following their fate during the embryonic, perinatal, and adult neurogenesis in a variety of vertebrate species including birds, reptiles, and mammals. Due to BrdU toxicity, its incorporation into replicating DNA presents adverse consequences on the generation, survival, and settled patterns of cells. This may lead to false results and misinterpretation in the identification of proliferative neuroblasts. In this review, I will indicate the detrimental effects of this nucleoside during the development of the central nervous system, as well as the reliability of BrdU labeling to detect proliferating neuroblasts. Moreover, it will show factors influencing BrdU immunodetection and the contribution of this nucleoside to the study of prenatal, perinatal, and adult neurogenesis. Human adult neurogenesis will also be discussed. It is my hope that this review serves as a reference for those researchers who focused on detecting cells that are in the synthetic phase of the cell cycle.

Key words: 5-bromo-2′-deoxyuridine, adult neurogenesis, human adult neurogenesis, labeling, pitfalls, prenatal neurogenesis, proliferation, S-phase, suturing S-phase, toxicity