中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (7): 1575-1583.doi: 10.4103/1673-5374.387977

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

Runx2调控在许旺细胞迁移、再髓鞘化和损伤后周围神经再生过程中作用

  

  • 出版日期:2024-07-15 发布日期:2023-11-28

Runx2 regulates peripheral nerve regeneration to promote Schwann cell migration and re-myelination

Rong Hu1, *, Xinpeng Dun2, Lolita Singh3, Matthew C. Banton3   

  1. 1School of Traditional Chinese Medicine, Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China; 2The Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China; 3Faculty of Health, University of Plymouth, Plymouth, UK
  • Online:2024-07-15 Published:2023-11-28
  • Contact: Rong Hu, PhD, 522577026@qq.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 82104795 (to RH).

摘要:

Runx2转录是成骨细胞分化和功能的主要调控因子,但它在周围神经修复中的作用尚不清楚。实验分析了损伤后的 Runx2 调控,并确定其在许旺细胞中特异性上调。实验还利用许旺细胞特异性 Runx2 基因敲除小鼠,研究了Runx2在周围神经的发育和再生过程中的作用。发现Runx2 基因敲除小鼠坐骨神经损伤后再生过程的多个环节都依赖于Runx2。在 Runx2 基因敲除小鼠中观察到的变化包括许旺细胞增殖增加、许旺细胞迁移和轴突再生受损、轴突再髓鞘化减少以及再生后期巨噬细胞清除受阻。研究结果表明,Runx2 是调控周围神经修复的许旺细胞可塑性的关键调节因子。Runx2 在许旺细胞迁移、再髓鞘化和损伤后周围神经功能恢复中发挥着重要作用。

https://orcid.org/0000-0001-9219-4524 (Rong Hu)

Abstract: Runx2 is a major regulator of osteoblast differentiation and function; however, the role of Runx2 in peripheral nerve repair is unclear. Here, we analyzed Runx2 expression following injury and found that it was specifically up-regulated in Schwann cells. Furthermore, using Schwann cell-specific Runx2 knockout mice, we studied peripheral nerve development and regeneration and found that multiple steps in the regeneration process following sciatic nerve injury were Runx2-dependent. Changes observed in Runx2 knockout mice include increased proliferation of Schwann cells, impaired Schwann cell migration and axonal regrowth, reduced re-myelination of axons, and a block in macrophage clearance in the late stage of regeneration. Taken together, our findings indicate that Runx2 is a key regulator of Schwann cell plasticity, and therefore peripheral nerve repair. Thus, our study shows that Runx2 plays a major role in Schwann cell migration, re-myelination, and peripheral nerve functional recovery following injury.

Key words: macrophage clearance, migration, peripheral nerve injury, regeneration, re-myelination, Runx2, Schwann cells