中国神经再生研究(英文版) ›› 2025, Vol. 20 ›› Issue (11): 3193-3206.doi: 10.4103/NRR.NRR-D-24-00771

• 综述:退行性病与再生 • 上一篇    下一篇

诱导多能干细胞产生多巴胺能神经元治疗帕金森病

  

  • 出版日期:2025-11-15 发布日期:2025-02-22

Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease

Ling-Xiao Yi1, #, Hui Ren Woon2, #, Genevieve Saw1, #, Li Zeng1, 3, 4, Eng King Tan1, 3, 4, *, Zhi Dong Zhou1, 4, *   

  1. 1 National Neuroscience Institute, Singapore;  2 Nanyang Technology University, Singapore;  3 Department of Neurology, Singapore General Hospital, Singapore;  4 Signature Research Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore
  • Online:2025-11-15 Published:2025-02-22
  • Contact: Zhi Dong Zhou, MD, PhD, zhidong.zhou@duke-nus.edu.sg; Eng King Tan, MD, Tan.eng.king@sgh.com.sg
  • Supported by:
    This work was supported by Singapore National Medical Research Council (NMRC) grants, including CS-IRG, HLCA2022 (to ZDZ), STaR, OF LCG 000207 (to EKT), a Clinical Translational Research Programme in Parkinson’s Disease, as well as Duke-Duke-NUS collaboration pilot grant (to ZDZ).

摘要: https://orcid.org/0000-0003-0251-4163 (Zhi Dong Zhou); https://orcid.org/0000-0003-2977-9743 (Eng King Tan)

Abstract: The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson’s disease, the second most common human neurodegenerative disease. Although the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson’s disease is still unclear, the advancement of stem cell approaches has shown promise for Parkinson’s disease research and therapy. The induced pluripotent stem cells have been commonly used to generate dopaminergic neurons, which has provided valuable insights to improve our understanding of Parkinson’s disease pathogenesis and contributed to anti-Parkinson’s disease therapies. The current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem cells. The benefits of induced pluripotent stem cell-based research are highlighted. Various dopaminergic neuron differentiation protocols from induced pluripotent stem cells are compared. The emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are evaluated. Finally, limitations, challenges, and future directions of induced pluripotent stem cell– based approaches are analyzed and proposed, which will be significant to the future application of induced pluripotent stem cell–related techniques for Parkinson’s disease.

Key words: dopaminergic neurons, induced pluripotent stem cells, Parkinson’s disease, stem cell approaches