中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (10): 5013-5019.doi: 10.4103/NRR.NRR-D-25-00501

• 原著:退行性病与再生 • 上一篇    下一篇

枸杞糖肽减轻神经元β-淀粉样蛋白毒性

  

  • 出版日期:2026-10-15 发布日期:2026-06-15

Lycium barbarum glycopeptide protects neurons from amyloid-beta toxicity

Zihang Chen1, 2, #, Xiao Wang3, #, Simin Wang4, #, Zelin Wu1, Huajun Wang1, *, Kin Chiu2, *, Xiaofei Zheng1, *, Kwok-Fai So2, 3, *   

  1. 1Department of Sports Medicine, The First Affiliated Hospital, Guangdong Provincial Key Laboratory of Speed Capability, The Guangzhou Key Laboratory of Precision Orthopedics and Regenerative Medicine, Jinan University, Guangzhou, Guangdong Province, China; 
    2Department of Psychology, State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong Special Administrative Region, China; 
    3Guangdong-Hongkong-Macau Institute of Central Nervous System (CNS) Regeneration, Ministry of Education Central Nervous System (CNS) Regeneration Collaborative Joint Laboratory, Jinan University, Guangzhou, Guangdong Province, China; 
    4Department of Anesthesiology, Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine, Zhongshan, Guangdong Province, China
  • Online:2026-10-15 Published:2026-06-15
  • Contact: Kwok-Fai So, PhD, hrmaskf@hku.hk; Xiaofei Zheng, PhD, xfzheng@jnu.edu.cn; Kin Chiu, PhD, datwai@hku.hk; Huajun Wang, PhD, whj323@126.com.
  • Supported by:
    This study was supported by the Clinical Frontier Technology Program of the First Hospital of Jinan University, No. JNU1AF-CFTP-2022-a01204 (to XZ); Science and Technology Projects in Guangzhou, No. 2024A03 J0971 (to XZ); the Open Fund of Guangdong Provincial Key Laboratory of Spine and Spinal Cord Reconstruction, No. 2023B121203001 (to HW).

摘要:

神经元内淀粉样β蛋白寡聚体沉积及其相关的神经毒性是阿尔茨海默病发病的最直接指标之一。枸杞糖肽富含生物活性化合物,具有抗氧化、抗炎和神经保护作用,但其对神经元内淀粉样β积累的清除作用尚在研究中。实验显示,淀粉样β蛋白寡聚体可对N2A细胞产生毒性作用,导致活性氧水平升高及炎症酶诱导型一氧化氮合酶表达增加。枸杞糖肽通过减轻细胞损伤、增强细胞存活率、降低活性氧水平和诱导型一氧化氮合酶表达,以及上调抗氧化酶mRNA水平,包括超氧化物歧化酶1、超氧化物歧化酶2和谷胱甘肽过氧化物酶4来对抗这些效应。此外,枸杞糖肽激活PI3K/Akt/P70S6K信号通路并促进脑源性神经营养因子的表达,从而发挥神经保护作用。这些发现表明,枸杞糖肽可能是治疗阿尔茨海默病的潜在候选药物。


https://orcid.org/0000-0003-4039-4246 (Kwok-Fai So); 

https://orcid.org/0000-0001-7502-6131 (Xiaofei Zheng); 

https://orcid.org/0000-0001-6581-9007 (Kin Chiu);

https://orcid.org/0000-0001-6235-8997 (Huajun Wang)

关键词: 枸杞糖肽, 阿尔茨海默病, β-淀粉样蛋白, 氧化应激, 神经保护, 脑源性神经营养因子, PI3K/Akt/P70S6K信号通路, 活性氧, 神经再生

Abstract:

Oligomeric amyloid-beta deposition within neurons and its associated neurotoxicity are among the most direct indicators of the onset of Alzheimer’s disease. Investigations into strategies aimed at reducing amyloid-beta accumulation or promoting its clearance in neurons are currently being carried out. Lycium barbarum glycopeptide is rich in biologically active compounds and possesses antioxidant, anti-inflammatory, and neuroprotective properties. This study verified that the oligomer amyloid-beta1–42 induced toxic effects in mouse N2a neuroblastoma cells, resulting in elevated levels of reactive oxygen species and increased expression of the inflammatory enzyme inducible nitric oxide synthase. Lycium barbarum glycopeptide counteracted these effects by alleviating cell damage, enhancing cell viability, reducing the levels of reactive oxygen species and inducible nitric oxide synthase expression, and up-regulating the mRNA levels of antioxidant enzymes, including superoxide dismutase 1, superoxide dismutase 2, and glutathione peroxidase 4. Lycium barbarum glycopeptide also activated the phosphoinositide 3-kinase/Akt/p70S6K signaling pathway and promoted the expression of brain-derived neurotrophic factor, thus exerting neuroprotective effects. These findings indicate that Lycium barbarum glycopeptide may be a promising candidate for the treatment of Alzheimer’s disease.

Key words: Alzheimer’s disease, amyloid-beta, brain-derived neurotrophic factor, Lycium barbarum glycopeptide, N2A cells, neuroinflammation, neuroprotection, oxidative stress, PI3K/Akt/p70S6K signaling, reactive oxygen species