中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (20): 1534-1539.

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

Differential protein expression in spinal cord tissue of a rabbit model of spinal cord ischemia/reperfusion injury

  

  • 收稿日期:2012-03-06 修回日期:2012-06-10 出版日期:2012-07-15 发布日期:2012-07-15

Differential protein expression in spinal cord tissue of a rabbit model of spinal cord ischemia/reperfusion injury

Qi Gao, Yonghui Liang, Xiaoyu Yang, Guifeng Liu, Xiaoxue Li, Benqing Zhu, Jian Liu, Maoguang Yang, Weiwei Xia, Jian Dong, Jianhang Jiao   

  1. Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China
  • Received:2012-03-06 Revised:2012-06-10 Online:2012-07-15 Published:2012-07-15
  • Contact: Xiaoyu Yang, Doctoral supervisor, Professor, Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China; Guifeng Liu, Studying for doctorate, Attending physician, Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China yang-xiaoyu88@sina.com; liuguifeng1011@sina.com
  • About author:Qi Gao☆, Studying for doctorate, Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China Qi Gao and Yonghui Liang contributed equally to this paper.

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Abstract:

New Zealand rabbits were randomly divided into an ischemia group (occlusion of the abdominal aorta for 60 minutes), an ischemia-reperfusion group (occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group. Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/ reperfusion groups and 23 of them were identified by mass spectrometry. In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated. In the ischemia/reperfusion group, the expression of four proteins was up regulated, and that of two proteins was down regulated. In the sham-surgery group, only one protein was detected. In the ischemia and ischemia/reperfusion groups, four proteins overlapped between groups with the same differential expression, including three that were up regulated and one down regulated. These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.

Key words: spinal cord injury, ischemia/reperfusion, two-dimensional gel electrophoresis, mass spectrometry, rabbit, proteomics, neural regeneration