中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (11): 2429-2430.doi: 10.4103/1673-5374.335809

• 观点:脑损伤修复保护与再生 • 上一篇    下一篇

利用多光子成像和综合清除揭示神经损伤后原位神经免疫相互作用的性别差异

  

  • 出版日期:2022-11-15 发布日期:2022-04-22

Utilizing multiphoton imaging and integrative clearing to reveal sex differences in neuroimmune interactions after nerve injury

Zachary W. Castillo, Michael D. Burton*   

  1. Neuroimmunology and Behavior Laboratory, Department of Neuroscience, School of Behavioral and Brain Sciences, Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, USA
  • Online:2022-11-15 Published:2022-04-22
  • Contact: Michael D. Burton, PhD, Michael.Burton@utdallas.edu.
  • Supported by:
    This work was supported by NIH grant K22NS096030 (to MDB), American Pain Society Future Leaders Grant (MDB), Rita Allen Foundation Award in Pain (to MDB), and The University of Texas System STARS program research support grant (to MDB).

摘要: https://orcid.org/0000-0002-0628-824X (Michael D. Burton) 
https://orcid.org/0000-0002-0993-5318 (Zachary W. Castillo)

Abstract: With the constant development of multiphoton microscopy, our ability to observe complex and dynamic biological processes deeper within living tissue, is steadily improving. Researchers use multiphoton microscopy, because experiments can be conducted with little to no invasiveness or tissue damage over a long period of time with no photodamage (Mancuso et al., 2009). This allows for the introduction of tissue into the context of a three-dimensional (3D) environment in which visualization of cellular activation and interaction is viable. By circumventing a distorted reconstruction with limited z-stacks, multiphoton imaging provides enhanced spatiotemporal resolution.