中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (1): 207-212.doi: 10.4103/1673-5374.344836

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

电沉积法制备壳聚糖/氧化石墨烯导管:修复周围神经缺损的潜在策略

  

  • 出版日期:2023-01-15 发布日期:2022-06-17
  • 基金资助:

    国家自然科学基金(81871493);武汉大学医学部医学腾飞计划项目(TFLC2018003)

Electrodeposition of chitosan/graphene oxide conduit to enhance peripheral nerve regeneration

Ya-Nan Zhao1, 2, #, Ping Wu1, #, Zi-Yuan Zhao3, Fei-Xiang Chen1, Ao Xiao1, Zhi-Yi Yue1, Xin-Wei Han2, Yong Zheng1, *, Yun Chen1, *   

  1. 1Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Disease, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, China; 2Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China; 3Clinical Medical College, Hubei University of Science and Technology, Xianning, Hubei Province, China
  • Online:2023-01-15 Published:2022-06-17
  • Contact: Yong Zheng, PhD, zhengyong@whu.edu.cn; Yun Chen, PhD, yunchen@whu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81871493 (to YC) and the Medical Science Advancement Program (Clinical Medicine) of Wuhan University, No. TFLC2018003 (to YC).

摘要:

目前,商品化的神经导管已被应用于周围神经缺损的修复;然而,具备良好生物相容性的导管仍有待进一步开发。实验应用电沉积技术制备了一系列壳聚糖/氧化石墨烯(GO)复合神经导管(CHGF-nn=00.250.51n为混合溶液中GO的浓度(wt%))。CHGF-n许旺细胞的增殖和黏附能力影响的评估结果显示,许旺细胞在复合神经导管表面具有良好的黏附和增殖能力,神经再生相关因子,如Krox20(髓鞘化关键因子)、Zeb2(对雪旺细胞分化、髓鞘化和神经修复至关重要)和TGF-β(一种具有再生功能的细胞因子)表达也出现上调。实验还显示,GO含量为0.25%的神经导管(CHGFC-0.25)修复10mm坐骨神经缺损的大鼠坐骨神经功能以及神经和腓肠肌的组织学表现均明显优于GO含量为0的神经导管,效果与自体移植组相似。总之,体外和体内实验联合证实了电沉积技术制备的壳聚糖/氧化石墨烯导管具备良好的生物相容性,因此可作为周围神经修复的候选策略。

https://orcid.org/0000-0002-5872-9609 (Yong Zheng); https://orcid.org/0000-0002-5984-7455 (Yun Chen)

Abstract: Currently available commercial nerve guidance conduits have been applied in the repair of peripheral nerve defects. However, a conduit exhibiting good biocompatibility remains to be developed. In this work, a series of chitosan/graphene oxide (GO) films with concentrations of GO varying from 0–1 wt% (collectively referred to as CHGF-n) were prepared by an electrodeposition technique. The effects of CHGF-n on proliferation and adhesion abilities of Schwann cells were evaluated. The results showed that Schwann cells exhibited elongated spindle shapes and upregulated expression of nerve regeneration-related factors such as Krox20 (a key myelination factor), Zeb2 (essential for Schwann cell differentiation, myelination, and nerve repair), and transforming growth factor β (a cytokine with regenerative functions). In addition, a nerve guidance conduit with a GO content of 0.25% (CHGFC-0.25) was implanted to repair a 10-mm sciatic nerve defect in rats. The results indicated improvements in sciatic functional index, electrophysiology, and sciatic nerve and gastrocnemius muscle histology compared with the CHGFC-0 group, and similar outcomes to the autograft group. In conclusion, we provide a candidate method for the repair of peripheral nerve defects using free-standing chitosan/GO nerve conduits produced by electrodeposition.

Key words: chitosan, electrodeposition, free-standing, graphene oxide, nerve conduit, nerve factors, Schwann cells, tissue engineerin