中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (3): 432-437.doi: 10.4103/1673-5374.153692

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

高眼压小鼠视网膜JNK3表达:减少神经节细胞凋亡的目标靶点?

  

  • 收稿日期:2015-01-19 出版日期:2015-03-20 发布日期:2015-03-20
  • 基金资助:

    四川省泸州市泸州医学院青年基金([2010]108号)四川省泸州市泸州医学院附属医院人才基金项目([2011]43号)

c-Jun N-terminal kinase 3 expression in the retina of ocular hypertension mice: a possible target to reduce ganglion cell apoptosis

Yue He 1, Jie Chen 2, Shu-guang Zhang 3, Yuan-sheng Yuan 4, Yan Li 4, Hong-bin Lv 1, Jin-hua Gan 1   

  1. 1 Department of Ophthalmology, the Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China
    2 Department of Rheumatology and Immunology, the Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China
    3 Department of Ophthalmology, the Second People’s Hospital of Zhengzhou City, Zhengzhou, Henan Province, China
    4 Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China
  • Received:2015-01-19 Online:2015-03-20 Published:2015-03-20
  • Contact: Yue He, M.D. Candidate, hylyfy@163.com.
  • Supported by:

    This work was supported by grants from the Youth Foundation of Luzhou Medical College of Luzhou City of Sichuan Province of China, No. [2010]108; the Talent Fund of the Affiliated Hospital of Luzhou Medical College of Luzhou City of Sichuan Province of China, No. [2011]43.

摘要:

青光眼是以视神经节细胞丢失为特征的一种视神经病变,在青光眼中JNK是否参与视网膜神经节细胞凋亡目前尚存在争议?为此,实验以减少房水激光灼烧方法建立高眼压模型小鼠,发现损伤后眼组织出现明显病理改变,且随眼压升高视网膜神经节细胞凋亡明显增加,而视网膜中JNK3 mRNA的表达也随之升高。提示视网膜中JNK3 mRNA表达增加与眼内压升高密切相关,也与视网膜神经节细胞凋亡呈正相关。

关键词: 神经再生, 高眼压, JNK3, 视网膜神经节细胞, 青光眼, 眼内压, 激光光凝

Abstract:

Glaucoma, a type of optic neuropathy, is characterized by the loss of retinal ganglion cells. It remains controversial whether c-Jun N-terminal kinase (JNK) participates in the apoptosis of retinal ganglion cells in glaucoma. This study sought to explore a possible mechanism of action of JNK signaling pathway in glaucoma-induced retinal optic nerve damage. We established a mouse model of chronic ocular hypertension by reducing the aqueous humor followed by photocoagulation using the laser ignition method. Results showed significant pathological changes in the ocular tissues after the injury. Apoptosis of retinal ganglion cells increased with increased intraocular pressure, as did JNK3 mRNA expression in the retina. These data indicated that the increased expression of JNK3 mRNA was strongly associated with the increase in intraocular pressure in the retina, and correlated positively with the apoptosis of retinal ganglion cells.

Key words: nerve regeneration, ocular hypertension, JNK3, retinal ganglion cell, glaucoma, laser photocoagulation, intraocular pressure, neural regeneration