中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (28): 2189-2198.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

端脑素激活ERM家族蛋白/磷脂酰肌醇-3-激酶/蛋白激酶B信号途径抑制淀粉样β蛋白诱导PAJU细胞的凋亡

  

  • 收稿日期:2012-03-18 修回日期:2012-06-13 出版日期:2012-10-05 发布日期:2012-10-05

Telencephalin protects PAJU cells from amyloid beta protein-induced apoptosis by activating the ezrin/radixin/moesin protein family/ phosphatidylinositol-3-kinase/protein kinase B pathway

Heping Yang 1, 2, 3, Dapeng Wu3, Xiaojie Zhang4, Xiang Wang3, Yi Peng3, Zhiping Hu3   

  1. 1 Department of Neurology, Shangrao No. 5 People’s Hospital, the Second Affiliated Hospital of Nanchang University, Shangrao 334000, Jiangxi Province, China
    2 Department of Neurology, Ruikang Hospital Affiliated to Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China
    3 Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China
    4 Department of Psychiatry, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China
  • Received:2012-03-18 Revised:2012-06-13 Online:2012-10-05 Published:2012-10-05
  • Contact: Hep-ing Yang, Department of Neurology, Shangrao No. 5 People’s Hospital, the Second Affiliated Hospital of Nanchang University, Shan-grao 334000, Jiangxi Prov-ince, China; Department of Neurology, Ruikang Hospital Affiliated to Guangxi Tradi-tional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China; Department of Neurology, Xiangya Second Hospital, Central South Uni-versity, Changsha 410011, Hunan Province, China Fenlenteng2004@126.com
  • About author:Heping Yang☆, Ph.D., Asso-ciate chief physician, De-partment of Neurology, Shangrao No. 5 People’s Hospital, the Second Affi-liated Hospital of Nanchang University, Shangrao 334000, Jiangxi Province, China; Department of Neu-rology, Ruikang Hospital Affiliated to Guangxi Tradi-tional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China; Department of Neurology, Xiangya Second Hospital, Central South Uni-versity, Changsha 410011, Hunan Province, China

Abstract:

Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidyli-nositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid, while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin acti-vates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis.

Key words: telencephalin/intercellular adhesion molecule 5, amyloid beta protein, ezrin/radixin/moesin family proteins/phosphatidylinositol-3-kinase/protein kinase B signal transduction, neural regeneration